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pBAD-DEST49
Gateway® destination vector for the regulated expression of N-terminally tagged HP-thioredoxin fusion proteins in bacterial cells.
Sequence Author: Thermo Fisher (Invitrogen)
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Plasmids
Gateway® Cloning Vectors
pBAD-DEST49
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NruI
(5868)
EcoRV
(5546)
NsiI
(5169)
SphI
(5167)
MauBI
(5145)
PfoI
(5077)
PflFI
-
Tth111I
(4978)
BsaAI
(4972)
NdeI
(4901)
PciI
(4722)
AhdI
(3834)
BglI
(3716)
NmeAIII
(3687)
AseI
(3659)
FspI
(3611)
PvuI
(3465)
BstEII
(77)
RBS
AleI
(441)
RsrII
(448)
HP-thioredoxin
BspDI
*
-
ClaI
*
(530)
NgoMIV
(673)
NaeI
(675)
EagI
-
NotI
(844)
PvuII
(1064)
EcoRI
(1164)
PasI
(1396)
BbvCI
(1889)
SrfI
(2035)
BmgBI
(2069)
BstXI
(2152)
BfuAI
-
BspMI
(2279)
PstI
(2290)
SalI
(2292)
BclI
*
(2422)
HindIII
(2427)
BseRI
(2448)
PmeI
(2511)
rrnB T1 terminator
rrnB T2 terminator
BstEII
(77)
BstEII
(77)
BstEII
AleI
(441)
AleI
(441)
AleI
RsrII
(448)
RsrII
(448)
RsrII
BspDI
*
-
(530)
BspDI
*
-
(530)
BspDI
*
-
ClaI
*
(530)
ClaI
*
(530)
ClaI
*
NgoMIV
(673)
NgoMIV
(673)
NgoMIV
NaeI
(675)
NaeI
(675)
NaeI
NotI
(844)
NotI
(844)
NotI
EagI
-
(844)
EagI
-
(844)
EagI
-
PvuII
(1064)
PvuII
(1064)
PvuII
EcoRI
(1164)
EcoRI
(1164)
EcoRI
PasI
(1396)
PasI
(1396)
PasI
BbvCI
(1889)
BbvCI
(1889)
BbvCI
SrfI
(2035)
SrfI
(2035)
SrfI
BmgBI
(2069)
BmgBI
(2069)
BmgBI
BstXI
(2152)
BstXI
(2152)
BstXI
BspMI
(2279)
BspMI
(2279)
BspMI
BfuAI
-
(2279)
BfuAI
-
(2279)
BfuAI
-
PstI
(2290)
PstI
(2290)
PstI
SalI
(2292)
SalI
(2292)
SalI
BclI
*
(2422)
BclI
*
(2422)
BclI
*
HindIII
(2427)
HindIII
(2427)
HindIII
BseRI
(2448)
BseRI
(2448)
BseRI
PmeI
(2511)
PmeI
(2511)
PmeI
PvuI
(3465)
PvuI
(3465)
PvuI
FspI
(3611)
FspI
(3611)
FspI
AseI
(3659)
AseI
(3659)
AseI
NmeAIII
(3687)
NmeAIII
(3687)
NmeAIII
BglI
(3716)
BglI
(3716)
BglI
AhdI
(3834)
AhdI
(3834)
AhdI
PciI
(4722)
PciI
(4722)
PciI
NdeI
(4901)
NdeI
(4901)
NdeI
BsaAI
(4972)
BsaAI
(4972)
BsaAI
Tth111I
(4978)
Tth111I
(4978)
Tth111I
PflFI
-
(4978)
PflFI
-
(4978)
PflFI
-
PfoI
(5077)
PfoI
(5077)
PfoI
MauBI
(5145)
MauBI
(5145)
MauBI
SphI
(5167)
SphI
(5167)
SphI
NsiI
(5169)
NsiI
(5169)
NsiI
EcoRV
(5546)
EcoRV
(5546)
EcoRV
NruI
(5868)
NruI
(5868)
NruI
HP-thioredoxin
HP-thioredoxin
HP-thioredoxin
HP-thioredoxin
rrnB T1 terminator
rrnB T1 terminator
rrnB T1 terminator
rrnB T1 terminator
rrnB T2 terminator
rrnB T2 terminator
rrnB T2 terminator
rrnB T2 terminator
RBS
RBS
RBS
RBS
pBAD-DEST49
6160 bp
NruI (5868)
1 site
T
C
G
C
G
A
A
G
C
G
C
T
EcoRV (5546)
1 site
G
A
T
A
T
C
C
T
A
T
A
G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NsiI (5169)
1 site
A
T
G
C
A
T
T
A
C
G
T
A
SphI (5167)
1 site
G
C
A
T
G
C
C
G
T
A
C
G
MauBI (5145)
1 site
C
G
C
G
C
G
C
G
G
C
G
C
G
C
G
C
PfoI (5077)
1 site
T
C
C
N
G
G
A
A
G
G
N
C
C
T
Sticky ends from different PfoI sites may not be compatible.
PflFI (4978)
1 site
G
A
C
N
N
N
G
T
C
C
T
G
N
N
N
C
A
G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I (4978)
1 site
G
A
C
N
N
N
G
T
C
C
T
G
N
N
N
C
A
G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsaAI (4972)
1 site
Y
A
C
G
T
R