pBAD-DEST49

Gateway® destination vector for the regulated expression of N-terminally tagged HP-thioredoxin fusion proteins in bacterial cells.

Sequence Author: Thermo Fisher (Invitrogen)

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HomePlasmidsGateway® Cloning VectorspBAD-DEST49
NruI (5868) EcoRV (5546) NsiI (5169) SphI (5167) MauBI (5145) PfoI (5077) PflFI - Tth111I (4978) BsaAI (4972) NdeI (4901) PciI (4722) AhdI (3834) BglI (3716) NmeAIII (3687) AseI (3659) FspI (3611) PvuI (3465) BstEII (77) RBS AleI (441) RsrII (448) HP-thioredoxin BspDI * - ClaI * (530) NgoMIV (673) NaeI (675) EagI - NotI (844) PvuII (1064) EcoRI (1164) PasI (1396) BbvCI (1889) SrfI (2035) BmgBI (2069) BstXI (2152) BfuAI - BspMI (2279) PstI (2290) SalI (2292) BclI * (2422) HindIII (2427) BseRI (2448) PmeI (2511) rrnB T1 terminator rrnB T2 terminator pBAD-DEST49 6160 bp
NruI  (5868)
1 site		 T C G C G A A G C G C T
EcoRV  (5546)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NsiI  (5169)
1 site		 A T G C A T T A C G T A
SphI  (5167)
1 site		 G C A T G C C G T A C G
MauBI  (5145)
1 site		 C G C G C G C G G C G C G C G C
PfoI  (5077)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
PflFI  (4978)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4978)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsaAI  (4972)
1 site		 Y A C G T R