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pcDNA-DEST47
Gateway® destination vector for expressing C-terminally GFP-tagged proteins in mammalian cells.
Sequence Author: Thermo Fisher (Invitrogen)
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Plasmids
Gateway® Cloning Vectors
pcDNA-DEST47
Show Static Map
SgrDI
(7778)
PvuI
(7227)
AhdI
(6857)
PciI
(5964)
PfoI
(5388)
RsrII
(5127)
PflFI
-
Tth111I
(4729)
PluTI
(4614)
SfoI
(4612)
NarI
(4611)
KasI
(4610)
AvrII
(4401)
StuI
(4400)
BseRI
(4397)
CsiI
-
SexAI
*
(4168)
BglII
(12)
SpeI
(249)
CMV enhancer
SnaBI
(590)
NotI
(1040)
lac UV5 promoter
EcoRI
(1360)
BsmBI
-
Esp3I
(1584)
PasI
-
PflMI
*
(1592)
BbvCI
(2084)
SrfI
(2230)
BmgBI
(2264)
BstXI
(2347)
NheI
(2631)
BmtI
(2635)
PaeR7I
-
PspXI
-
XhoI
(3051)
BstBI
(3252)
BbsI
(3564)
DraIII
(3878)
BglII
(12)
BglII
(12)
BglII
SpeI
(249)
SpeI
(249)
SpeI
SnaBI
(590)
SnaBI
(590)
SnaBI
NotI
(1040)
NotI
(1040)
NotI
EcoRI
(1360)
EcoRI
(1360)
EcoRI
Esp3I
(1584)
Esp3I
(1584)
Esp3I
BsmBI
-
(1584)
BsmBI
-
(1584)
BsmBI
-
PflMI
*
(1592)
PflMI
*
(1592)
PflMI
*
PasI
-
(1592)
PasI
-
(1592)
PasI
-
BbvCI
(2084)
BbvCI
(2084)
BbvCI
SrfI
(2230)
SrfI
(2230)
SrfI
BmgBI
(2264)
BmgBI
(2264)
BmgBI
BstXI
(2347)
BstXI
(2347)
BstXI
NheI
(2631)
NheI
(2631)
NheI
BmtI
(2635)
BmtI
(2635)
BmtI
XhoI
(3051)
XhoI
(3051)
XhoI
PaeR7I
-
(3051)
PaeR7I
-
(3051)
PaeR7I
-
PspXI
-
(3051)
PspXI
-
(3051)
PspXI
-
BstBI
(3252)
BstBI
(3252)
BstBI
BbsI
(3564)
BbsI
(3564)
BbsI
DraIII
(3878)
DraIII
(3878)
DraIII
CsiI
-
(4168)
CsiI
-
(4168)
CsiI
-
SexAI
*
(4168)
SexAI
*
(4168)
SexAI
*
BseRI
(4397)
BseRI
(4397)
BseRI
StuI
(4400)
StuI
(4400)
StuI
AvrII
(4401)
AvrII
(4401)
AvrII
KasI
(4610)
KasI
(4610)
KasI
NarI
(4611)
NarI
(4611)
NarI
SfoI
(4612)
SfoI
(4612)
SfoI
PluTI
(4614)
PluTI
(4614)
PluTI
Tth111I
(4729)
Tth111I
(4729)
Tth111I
PflFI
-
(4729)
PflFI
-
(4729)
PflFI
-
RsrII
(5127)
RsrII
(5127)
RsrII
PfoI
(5388)
PfoI
(5388)
PfoI
PciI
(5964)
PciI
(5964)
PciI
AhdI
(6857)
AhdI
(6857)
AhdI
PvuI
(7227)
PvuI
(7227)
PvuI
SgrDI
(7778)
SgrDI
(7778)
SgrDI
CMV enhancer
CMV enhancer
CMV enhancer
CMV enhancer
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
pcDNA™-DEST47
7780 bp
SgrDI (7778)
1 site
C
G
T
C
G
A
C
G
G
C
A
G
C
T
G
C
PvuI (7227)
1 site
C
G
A
T
C
G
G
C
T
A
G
C
AhdI (6857)
1 site
G
A
C
N
N
N
N
N
G
T
C
C
T
G
N
N
N
N
N
C
A
G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI (5964)
1 site
A
C
A
T
G
T
T
G
T
A
C
A
PciI is inhibited by nonionic detergents.
PfoI (5388)
1 site
T
C
C
N
G
G
A
A
G
G
N
C
C
T
Sticky ends from different PfoI sites may not be compatible.
RsrII (5127)
1 site
C
G
G
W