pLenti6.4 R4R2 V5-DEST

Lentiviral Gateway® destination vector for a micro-RNA (miRNA) expression cassette.

Sequence Author: Thermo Fisher (Invitrogen)

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HomePlasmidsGateway® Cloning VectorspLenti6.4 R4R2 V5-DEST
PciI (8512) AhdI (7624) DraIII (6484) AvrII (6043) SfiI (5996) PmlI (4889) PGK promoter EcoNI (942) MfeI (961) BstAPI (1225) AleI (1349) KflI (1706) HpaI (1936) lac UV5 promoter EcoRI (2396) PasI (2628) BstZ17I (2931) TspMI - XmaI (3265) SmaI - SrfI (3267) BfuAI - BspMI (3511) PstI (3522) PaeR7I - PspXI - XhoI (3679) PspOMI (3691) ApaI (3695) BstBI (3702) MluI (3746) PfoI (4045) SpeI (4624) pLenti6.4/R4R2/V5-DEST 8922 bp
PciI  (8512)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
AhdI  (7624)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DraIII  (6484)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
AvrII  (6043)
1 site		 C C T A G G G G A T C C
SfiI  (5996)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PmlI  (4889)
1 site		 C A C G T G G T G C A C