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pLenti6.4 R4R2 V5-DEST
Lentiviral Gateway® destination vector for a micro-RNA (miRNA) expression cassette.
Sequence Author: Thermo Fisher (Invitrogen)
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pLenti6.4 R4R2 V5-DEST
Show Static Map
PciI
(8512)
AhdI
(7624)
DraIII
(6484)
AvrII
(6043)
SfiI
(5996)
PmlI
(4889)
PGK promoter
EcoNI
(942)
MfeI
(961)
BstAPI
(1225)
AleI
(1349)
KflI
(1706)
HpaI
(1936)
lac UV5 promoter
EcoRI
(2396)
PasI
(2628)
BstZ17I
(2931)
TspMI
-
XmaI
(3265)
SmaI
-
SrfI
(3267)
BfuAI
-
BspMI
(3511)
PstI
(3522)
PaeR7I
-
PspXI
-
XhoI
(3679)
PspOMI
(3691)
ApaI
(3695)
BstBI
(3702)
MluI
(3746)
PfoI
(4045)
SpeI
(4624)
EcoNI
(942)
EcoNI
(942)
EcoNI
MfeI
(961)
MfeI
(961)
MfeI
BstAPI
(1225)
BstAPI
(1225)
BstAPI
AleI
(1349)
AleI
(1349)
AleI
KflI
(1706)
KflI
(1706)
KflI
HpaI
(1936)
HpaI
(1936)
HpaI
EcoRI
(2396)
EcoRI
(2396)
EcoRI
PasI
(2628)
PasI
(2628)
PasI
BstZ17I
(2931)
BstZ17I
(2931)
BstZ17I
TspMI
-
(3265)
TspMI
-
(3265)
TspMI
-
XmaI
(3265)
XmaI
(3265)
XmaI
SmaI
-
(3267)
SmaI
-
(3267)
SmaI
-
SrfI
(3267)
SrfI
(3267)
SrfI
BspMI
(3511)
BspMI
(3511)
BspMI
BfuAI
-
(3511)
BfuAI
-
(3511)
BfuAI
-
PstI
(3522)
PstI
(3522)
PstI
PspXI
-
(3679)
PspXI
-
(3679)
PspXI
-
PaeR7I
-
(3679)
PaeR7I
-
(3679)
PaeR7I
-
XhoI
(3679)
XhoI
(3679)
XhoI
PspOMI
(3691)
PspOMI
(3691)
PspOMI
ApaI
(3695)
ApaI
(3695)
ApaI
BstBI
(3702)
BstBI
(3702)
BstBI
MluI
(3746)
MluI
(3746)
MluI
PfoI
(4045)
PfoI
(4045)
PfoI
SpeI
(4624)
SpeI
(4624)
SpeI
PmlI
(4889)
PmlI
(4889)
PmlI
SfiI
(5996)
SfiI
(5996)
SfiI
AvrII
(6043)
AvrII
(6043)
AvrII
DraIII
(6484)
DraIII
(6484)
DraIII
AhdI
(7624)
AhdI
(7624)
AhdI
PciI
(8512)
PciI
(8512)
PciI
PGK promoter
PGK promoter
PGK promoter
PGK promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
pLenti6.4/R4R2/V5-DEST
8922 bp
PciI (8512)
1 site
A
C
A
T
G
T
T
G
T
A
C
A
PciI is inhibited by nonionic detergents.
AhdI (7624)
1 site
G
A
C
N
N
N
N
N
G
T
C
C
T
G
N
N
N
N
N
C
A
G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DraIII (6484)
1 site
C
A
C
N
N
N
G
T
G
G
T
G
N
N
N
C
A
C
Sticky ends from different DraIII sites may not be compatible.
AvrII (6043)
1 site
C
C
T
A
G
G
G
G
A
T
C
C
SfiI (5996)
1 site
G
G
C
C
N
N
N
N
N
G
G
C
C
C
C
G
G
N
N
N
N
N
C
C
G
G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PmlI (4889)
1 site
C
A
C
G
T
G
G
T
G
C
A
C