pEF-DEST51

Gateway® destination vector for high-level constitutive expression of C-terminally V5- and 6xHis-tagged proteins in mammalian cells.

Sequence Author: Thermo Fisher (Invitrogen)

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ZraI (119) FspI (6885) AhdI (6663) BspQI - SapI (5654) PfoI (5194) BlpI (5098) PflFI - Tth111I (4977) PpuMI (4862) AvrII (4574) CsiI - SexAI * (4341) PmeI (3525) SgrDI (120) AatII (121) AflII (854) FseI (1179) Eco53kI (1301) SacI (1303) KasI (1413) NarI (1414) SfoI (1415) PluTI (1417) PaeR7I - XhoI (1440) T7 promoter NotI (1846) BspEI (2162) EcoRI (2166) BsmBI - Esp3I (2390) PflMI * (2398) BssHII (2660) SrfI (3037) BmgBI (3071) BstXI (3154) attR2 XbaI * (3428) BstBI (3445) V5 tag 6xHis pEF-DEST51 7464 bp