pET-62-DEST

Gateway® Nova bacterial destination vector for expressing proteins tagged at the C-terminus with 6xHis and Strep-Tag II.

Sequence Author: MilliporeSigma (Novagen)

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AvrII (6727) PacI (6723) PaeR7I - PspXI - XhoI (6711) BstBI (6692) 6xHis SalI (6520) PstI (6518) BfuAI - BspMI (6507) BmgBI (6297) SmaI - SrfI (6263) TspMI - XmaI (6261) BbvCI (6117) PasI (5624) EcoRI (5392) EagI - NotI (5072) XbaI (4916) lac operator T7 promoter BspDI * - ClaI * (4849) SgrAI (4805) BtgI (4687) SphI (4657) EcoNI (4592) BstAPI (4448) BclI * (4110) BstEII (3942) ApaI (3921) PspOMI (3917) HpaI (3622) BlpI (6745) DraIII (245) PsiI (370) AhdI (655) BsaI (716) BglI (775) FspI (877) PvuI (1025) PciI (2260) BspQI - SapI (2377) PflFI - Tth111I (2518) PpuMI (3255) pET-62-DEST™ 6828 bp
AvrII  (6727)
1 site
C C T A G G G G A T C C
PacI  (6723)
1 site
T T A A T T A A A A T T A A T T
PaeR7I  (6711)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (6711)
1 site
V C T C G A G B B G A G C T C V
XhoI  (6711)
1 site
C T C G A G G A G C T C
BstBI  (6692)
1 site
T T C G A A A A G C T T
SalI  (6520)
1 site
G T C G A C C A G C T G
PstI  (6518)
1 site
C T G C A G G A C G T C
BfuAI  (6507)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (6507)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BmgBI  (6297)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
SmaI  (6263)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
SrfI  (6263)
1 site
G C C C G G G C C G G G C C C G
TspMI  (6261)
1 site
C C C G G G G G G C C C
XmaI  (6261)
1 site
C C C G G G G G G C C