Gateway® Nova bacterial destination vector for expressing proteins tagged at the C-terminus with 6xHis and Strep-Tag II.
Explore Over 2.7k Plasmids: Gateway® Cloning Vectors | More Plasmid Sets
PaeR7I does not recognize the sequence CTCTCGAG.
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C.
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
SmaI can be used at 37°C for brief incubations.
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
Sticky ends from different PasI sites may not be compatible.