pET-57-DEST

Gateway® Nova bacterial destination vector for expressing proteins tagged at the N-terminus with NusA and at the C-terminus with Strep-Tag II.

Sequence Author: MilliporeSigma (Novagen)

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Strep-Tag II SalI (8088) PstI (8086) BmgBI (7865) SmaI - SrfI (7831) AvaI - BsoBI - TspMI - XmaI (7829) BbvCI (7685) PasI (7192) EcoRI (6960) EagI - NotI (6640) PmlI (6502) thrombin site SacI (6492) Eco53kI (6490) BglII (6369) StuI (6188) AatII (5132) ZraI (5130) BmtI (5000) NheI (4996) NdeI (4991) 6xHis ATG RBS XbaI (4916) T7 promoter SphI (4657) EcoNI (4592) AvrII (8261) DraIII (245) PsiI (370) BsaI (716) BglI (775) FspI (877) PciI (2260) BspQI - SapI (2377) PflFI - Tth111I (2518) PpuMI (3255) HpaI (3622) PspOMI (3917) ApaI (3921) BstEII (3942) pET-57-DEST™ 8362 bp