pET-57-DEST

Gateway® Nova bacterial destination vector for expressing proteins tagged at the N-terminus with NusA and at the C-terminus with Strep-Tag II.

Sequence Author: MilliporeSigma (Novagen)

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Strep-Tag II SalI (8088) PstI (8086) BmgBI (7865) SmaI - SrfI (7831) AvaI - BsoBI - TspMI - XmaI (7829) BbvCI (7685) PasI (7192) EcoRI (6960) EagI - NotI (6640) PmlI (6502) thrombin site SacI (6492) Eco53kI (6490) BglII (6369) StuI (6188) AatII (5132) ZraI (5130) BmtI (5000) NheI (4996) NdeI (4991) 6xHis ATG RBS XbaI (4916) T7 promoter SphI (4657) EcoNI (4592) AvrII (8261) DraIII (245) PsiI (370) BsaI (716) BglI (775) FspI (877) PciI (2260) BspQI - SapI (2377) PflFI - Tth111I (2518) PpuMI (3255) HpaI (3622) PspOMI (3917) ApaI (3921) BstEII (3942) pET-57-DEST™ 8362 bp
SalI  (8088)
1 site		 G T C G A C C A G C T G
PstI  (8086)
1 site		 C T G C A G G A C G T C
BmgBI  (7865)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
SmaI  (7831)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
SrfI  (7831)
1 site		 G C C C G G G C C G G G C C C G
AvaI  (7829)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (7829)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
TspMI  (7829)
1 site		 C C C G G G G G G C C C
Xm