pHELLSGATE

High-throughput binary Gateway® vector for silencing genes in plants using intron-containing hairpin RNA.
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AgeI (18,257) XbaI (17,913) XbaI (16,296) HindIII (16,284) BspDI - ClaI (16,279) PacI (16,168) KpnI (14,653) Acc65I (14,649) AbsI - XhoI (14,637) XhoI (13,020) StuI (12,234) SacI (11,646) Eco53kI (11,644) PmeI (11,220) BsiWI (11,055) FseI (10,595) Bsu36I (330) RsrII (1119) AflII (1923) MauBI (2118) LB T-DNA repeat AleI (5445) SexAI * (5533) FspAI (6101) NruI (6598) pHELLSGATE 18,691 bp
AgeI  (18,257)
1 site
A C C G G T T G G C C A
XbaI  (17,913)
2 sites
T C T A G A A G A T C T
XbaI  (16,296)
2 sites
T C T A G A A G A T C T
HindIII  (16,284)
1 site
A A G C T T T T C G A A
BspDI  (16,279)
1 site
A T C G A T T A G C T A
ClaI  (16,279)
1 site
A T C G A T T A G C T A
PacI  (16,168)
1 site
T T A A T T A A A A T T A A T T
KpnI  (14,653)
1 site
G G T A C C C C A T G G
Acc65I  (14,649)
1 site
G G T A C C C C A T G G
AbsI  (14,637)
1 site
C C T C G A G G G G A G C T C C
XhoI  (14,637)
2 sites
C T C G A G G A G C T C
XhoI  (13,020)
2 sites
C T C G A G G A G C T C
StuI  (12,234)
1 site
A G G C C T T C C G G A
SacI  (11,646)
1 site
G A G C T C C T C G A G
Eco53kI  (11,644)
1 site
G A G C T C C T C G A G
PmeI  (11,220)
1 site
G T T T A A A C C A A A T T T G
BsiWI  (11,055)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
FseI  (10,595)
1 site
G G C C G G