pYES-DEST52
Gateway® destination vector for galactose-inducible high-level expression of proteins in Saccharomyces cerevisiae.
Sequence Author: Thermo Fisher (Invitrogen)
Explore Over 2.7k Plasmids: Gateway® Cloning Vectors | More Plasmid Sets
No matches
| ||
Efficient cleavage requires at least two copies of the NaeI recognition sequence. |
| ||
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. |
| ||
SwaI is typically used at 25°C, but is 50% active at 37°C. |
|
|
| ||
| * Blocked by Dcm methylation. Sticky ends from different PfoI sites may not be compatible. |
|
| ||
Sticky ends from different BlpI sites may not be compatible. |
|
|
|
|
| ||
Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
| ||
Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
|
| ||
The 1-base overhangs produced by XcmI may be hard to ligate.Sticky ends from different XcmI sites may not be compatible. |
| ||
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
| ||
BsaHI is typically used at 37°C, but is even more active at 60°C. |
| ||
Sticky ends from different BglI sites may not be compatible. |
|
| ||
Efficient cleavage requires at least two copies of the SacII recognition sequence. |
|
|
| ||
Sticky ends from different PasI sites may not be compatible. |
| ||
| * Blocked by Dcm methylation. Sticky ends from different PflMI sites may not be compatible. |
| ||
BssHII is typically used at 50°C, but is 75% active at 37°C. |
|
|
| ||
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
| ||
SmaI can be used at 37°C for brief incubations. |
|
| ||
| * Blocked by Dam methylation. |
| ||
Sticky ends from different BstXI sites may not be compatible. |
|
|
| ||
The 1-base overhangs produced by BciVI may be hard to ligate.Sticky ends from different BciVI sites may not be compatible. |
|
| ||
The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
| 2μ ori 6200 .. 7080 = 881 bp yeast 2μ plasmid origin of replication |
| 2μ ori 6200 .. 7080 = 881 bp yeast 2μ plasmid origin of replication |
| AmpR 3621 .. 4481 = 861 bp 286 amino acids = 31.7 kDa 2 segments Segment 2: 3621 .. 4412 = 792 bp 263 amino acids = 29.1 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 3621 .. 4481 = 861 bp 286 amino acids = 31.7 kDa 2 segments Segment 1: signal sequence 4413 .. 4481 = 69 bp 23 amino acids = 2.6 kDa Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| AmpR 3621 .. 4481 = 861 bp 286 amino acids = 31.7 kDa 2 segments Product: β-lactamase confers resistance to ampicillin, carbenicillin, and related antibiotics |
| URA3 4577 .. 5380 = 804 bp 267 amino acids = 29.3 kDa Product: orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA) |
| URA3 4577 .. 5380 = 804 bp 267 amino acids = 29.3 kDa Product: orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA) |
| CmR 771 .. 1430 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
| CmR 771 .. 1430 = 660 bp 219 amino acids = 25.7 kDa Product: chloramphenicol acetyltransferase confers resistance to chloramphenicol |
| ori 2862 .. 3450 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| ori 2862 .. 3450 = 589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication |
| f1 ori 7149 .. 7604 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| f1 ori 7149 .. 7604 = 456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis |
| GAL1 promoter 2 .. 443 = 442 bp inducible promoter, regulated by Gal4 |
| GAL1 promoter 2 .. 443 = 442 bp inducible promoter, regulated by Gal4 |