pYES-DEST52
Gateway® destination vector for galactose-inducible high-level expression of proteins in Saccharomyces cerevisiae.
Sequence Author: Thermo Fisher (Invitrogen)
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Efficient cleavage requires at least two copies of the NaeI recognition sequence. |
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Efficient cleavage requires at least two copies of the NgoMIV recognition sequence. |
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SwaI is typically used at 25°C, but is 50% active at 37°C. |
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| * Blocked by Dcm methylation. Sticky ends from different PfoI sites may not be compatible. |
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Sticky ends from different BlpI sites may not be compatible. |
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
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Efficient cleavage requires at least two copies of the BsgI recognition sequence. Sticky ends from different BsgI sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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The 1-base overhangs produced by XcmI may be hard to ligate.Sticky ends from different XcmI sites may not be compatible. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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BsaHI is typically used at 37°C, but is even more active at 60°C. |
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Sticky ends from different BglI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SacII recognition sequence. |
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Sticky ends from different PasI sites may not be compatible. |
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| * Blocked by Dcm methylation. Sticky ends from different PflMI sites may not be compatible. |
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BssHII is typically used at 50°C, but is 75% active at 37°C. |
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Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
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SmaI can be used at 37°C for brief incubations. |
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| * Blocked by Dam methylation. |
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Sticky ends from different BstXI sites may not be compatible. |
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