pT-REx-DEST31

Gateway® destination vector enabling N-terminal 6xHis-tagging and tetracycline-inducible high-level expression.

Sequence Author: Thermo Fisher (Invitrogen)

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PciI (7164) AhdI (6276) XmnI (5676) PfoI (5237) BstBI (4982) RsrII (4816) PflFI - Tth111I (4418) PluTI (4303) SfoI (4301) NarI (4300) KasI (4299) AflII (4154) AvrII (4106) StuI (4105) BseRI (4102) SfiI (4059) CsiI - SexAI * (3873) KpnI (3760) CMV enhancer SnaBI (288) tet operator BglII (534) SgrDI (560) BbsI (636) ATG 6xHis AgeI - SgrAI (691) AleI (692) EcoRV (699) PaeR7I - PspXI - XhoI (703) EcoRI (1161) PasI - PflMI * (1393) BstZ17I (1696) BbvCI (1886) TspMI - XmaI (2030) SmaI - SrfI (2032) BmgBI (2066) XbaI (2433) PspOMI (2439) ApaI (2443) T7 promoter NheI (2547) BmtI (2551) BclI * (2823) MfeI (2916) HpaI (2929) DraIII (3423) Acc65I (3756)