pLenti6.2 C-Lumio V5-DEST

Lentiviral Gateway® destination vector for C-terminal tagging of a protein with the Lumio™ tetracysteine tag.

Sequence Author: Thermo Fisher (Invitrogen)

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PciI (8425) AhdI (7537) DraIII (6397) NaeI (6291) NgoMIV (6289) AvrII (5956) SfiI (5909) PmlI (4802) PGK promoter EcoNI (942) MfeI (961) BstAPI (1225) AleI (1349) KflI (1706) BspDI - ClaI (1795) CMV enhancer NdeI (1989) SnaBI (2095) EcoRV (2435) lac UV5 promoter PasI - PflMI * (3118) BstZ17I (3421) TspMI - XmaI (3755) SmaI - SrfI (3757) BfuAI - BspMI (4001) SalI (4014) HpaI (4154) MluI (4197) tetracysteine tag pLenti6.2/C-Lumio™/V5-DEST 8835 bp
PciI  (8425)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AhdI  (7537)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DraIII  (6397)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NaeI  (6291)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (6289)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
AvrII  (5956)
1 site
C C T A G G G G A T C C
SfiI  (5909)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PmlI  (4802)
1 site
C A C G T G G T G C A C
EcoNI  (942)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
MfeI  (961)
1 site
C A A T T G G T T A A C
BstAPI  (1225)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
AleI  (1349)
1 site
C A