pET-59-DEST

Gateway® Nova bacterial destination vector for expressing proteins tagged at the N-terminus with thioredoxin and at the C-terminus with Strep-Tag II.

Sequence Author: MilliporeSigma (Novagen)

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AvrII (7097) BstBI (7078) Strep-Tag II SalI (6924) PstI (6922) BfuAI - BspMI (6911) BmgBI (6701) SmaI - SrfI (6667) TspMI - XmaI (6665) BbvCI (6521) PasI (6028) EcoRI (5796) EagI - NotI (5476) PmlI (5338) thrombin site SacI (5328) Eco53kI (5326) RsrII (5095) BmtI (5000) NheI (4996) NdeI (4991) 6xHis ATG NcoI (4955) RBS XbaI (4916) T7 promoter SgrAI (4805) SphI (4657) EcoNI (4592) BstAPI (4448) BclI * (4110) BstEII (3942) ApaI (3921) PspOMI (3917) BlpI (7115) DraIII (245) PsiI (370) AhdI (655) BsaI (716) BglI (775) FspI (877) PvuI (1025) PciI (2260) BspQI - SapI (2377) PflFI - Tth111I (2518) PpuMI (3255) HpaI (3622) pET-59-DEST™ 7198 bp