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pET-59-DEST
Gateway® Nova bacterial destination vector for expressing proteins tagged at the N-terminus with thioredoxin and at the C-terminus with Strep-Tag II.
Sequence Author: MilliporeSigma (Novagen)
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Plasmids
Gateway® Cloning Vectors
pET-59-DEST
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AvrII
(7097)
BstBI
(7078)
Strep-Tag II
SalI
(6924)
PstI
(6922)
BfuAI
-
BspMI
(6911)
BmgBI
(6701)
SmaI
-
SrfI
(6667)
TspMI
-
XmaI
(6665)
BbvCI
(6521)
PasI
(6028)
EcoRI
(5796)
EagI
-
NotI
(5476)
PmlI
(5338)
thrombin site
SacI
(5328)
Eco53kI
(5326)
RsrII
(5095)
BmtI
(5000)
NheI
(4996)
NdeI
(4991)
6xHis
ATG
NcoI
(4955)
RBS
XbaI
(4916)
T7 promoter
SgrAI
(4805)
SphI
(4657)
EcoNI
(4592)
BstAPI
(4448)
BclI
*
(4110)
BstEII
(3942)
ApaI
(3921)
PspOMI
(3917)
BlpI
(7115)
DraIII
(245)
PsiI
(370)
AhdI
(655)
BsaI
(716)
BglI
(775)
FspI
(877)
PvuI
(1025)
PciI
(2260)
BspQI
-
SapI
(2377)
PflFI
-
Tth111I
(2518)
PpuMI
(3255)
HpaI
(3622)
DraIII
(245)
DraIII
(245)
DraIII
PsiI
(370)
PsiI
(370)
PsiI
AhdI
(655)
AhdI
(655)
AhdI
BsaI
(716)
BsaI
(716)
BsaI
BglI
(775)
BglI
(775)
BglI
FspI
(877)
FspI
(877)
FspI
PvuI
(1025)
PvuI
(1025)
PvuI
PciI
(2260)
PciI
(2260)
PciI
SapI
(2377)
SapI
(2377)
SapI
BspQI
-
(2377)
BspQI
-
(2377)
BspQI
-
Tth111I
(2518)
Tth111I
(2518)
Tth111I
PflFI
-
(2518)
PflFI
-
(2518)
PflFI
-
PpuMI
(3255)
PpuMI
(3255)
PpuMI
HpaI
(3622)
HpaI
(3622)
HpaI
PspOMI
(3917)
PspOMI
(3917)
PspOMI
ApaI
(3921)
ApaI
(3921)
ApaI
BstEII
(3942)
BstEII
(3942)
BstEII
BclI
*
(4110)
BclI
*
(4110)
BclI
*
BstAPI
(4448)
BstAPI
(4448)
BstAPI
EcoNI
(4592)
EcoNI
(4592)
EcoNI
SphI
(4657)
SphI
(4657)
SphI
SgrAI
(4805)
SgrAI
(4805)
SgrAI
XbaI
(4916)
XbaI
(4916)
XbaI
NcoI
(4955)
NcoI
(4955)
NcoI
NdeI
(4991)
NdeI
(4991)
NdeI
NheI
(4996)
NheI
(4996)
NheI
BmtI
(5000)
BmtI
(5000)
BmtI
RsrII
(5095)
RsrII
(5095)
RsrII
Eco53kI
(5326)
Eco53kI
(5326)
Eco53kI
SacI
(5328)
SacI
(5328)
SacI
PmlI
(5338)
PmlI
(5338)
PmlI
NotI
(5476)
NotI
(5476)
NotI
EagI
-
(5476)
EagI
-
(5476)
EagI
-
EcoRI
(5796)
EcoRI
(5796)
EcoRI
PasI
(6028)
PasI
(6028)
PasI
BbvCI
(6521)
BbvCI
(6521)
BbvCI
XmaI
(6665)
XmaI
(6665)
XmaI
TspMI
-
(6665)
TspMI
-
(6665)
TspMI
-
SrfI
(6667)
SrfI
(6667)
SrfI
SmaI
-
(6667)
SmaI
-
(6667)
SmaI
-
BmgBI
(6701)
BmgBI
(6701)
BmgBI
BfuAI
-
(6911)
BfuAI
-
(6911)
BfuAI
-
BspMI
(6911)
BspMI
(6911)
BspMI
PstI
(6922)
PstI
(6922)
PstI
SalI
(6924)
SalI
(6924)
SalI
BstBI
(7078)
BstBI
(7078)
BstBI
AvrII
(7097)
AvrII
(7097)
AvrII
BlpI
(7115)
BlpI
(7115)
BlpI
ATG
ATG
ATG
ATG
6xHis
6xHis
6xHis
6xHis
thrombin site
thrombin site
thrombin site
thrombin site
Strep-Tag II
Strep-Tag II
Strep-Tag II
Strep-Tag II
T7 promoter
T7 promoter
T7 promoter
T7 promoter
RBS
RBS
RBS
RBS
pET-59-DEST™
7198 bp