pLenti6 BLOCK-iT-DEST

Lentiviral Gateway® destination vector for an shRNA cassette from a U6 entry vector.

Sequence Author: Thermo Fisher (Invitrogen)

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KasI (413) PciI (8265) DraIII (6237) NaeI (6131) NgoMIV (6129) SfiI (5749) KpnI (5129) Acc65I (5125) BlpI (5106) PmlI (4642) BclI * (4633) SV40 promoter CsiI - SexAI * (4349) NarI (414) SfoI (415) PluTI (417) EcoNI (942) MfeI (961) AleI (1349) KflI (1706) NheI (1797) BmtI (1801) AfeI (1802) SpeI (1827) BspDI - ClaI (1834) HpaI (1843) BfuAI - BspMI (2394) BmgBI (2608) SrfI (2642) BstZ17I (2978) BamHI (3056) BspEI (3513) PaeR7I - PspXI - XhoI (4157) XbaI (4163) PspOMI (4169) ApaI (4173) SacII (4176) MluI (4224) AgeI (4230) pLenti6/BLOCK-iT™-DEST 8675 bp
KasI  (413)
1 site
G G C G C C C C G C G G
PciI  (8265)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
DraIII  (6237)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NaeI  (6131)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (6129)
1 site
G C C G G C C G G C C G