pT-REx-DEST30

Gateway® destination vector enabling tetracycline-inducible high-level expression.

Sequence Author: Thermo Fisher (Invitrogen)

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CMV enhancer PciI (7149) AhdI (6261) XmnI (5661) PfoI (5222) BstBI (4967) RsrII (4801) PflFI - Tth111I (4403) PluTI (4288) SfoI (4286) NarI (4285) KasI (4284) AflII (4139) AvrII (4091) StuI (4090) BseRI (4087) SfiI (4044) CsiI - SexAI * (3858) KpnI (3745) SnaBI (288) tet operator tet operator SgrDI (560) BbsI (636) AgeI (674) EcoRV (682) PaeR7I - PspXI - XhoI (686) EcoRI (1145) PasI - PflMI * (1377) BstZ17I (1680) BbvCI (1870) TspMI - XmaI (2014) SmaI - SrfI (2016) BmgBI (2050) PspOMI (2424) ApaI (2428) MluI (2436) T7 promoter NheI (2532) BmtI (2536) BclI * (2808) MfeI (2901) HpaI (2914) DraIII (3408) Acc65I (3741) pT-REx™-DEST30 7544 bp