pDEST10
Gateway® destination vector for expressing N-terminally 6xHis-tagged proteins in insect cells using the Bac-to-Bac® baculovirus system.
Sequence Author: Thermo Fisher (Invitrogen)
Explore Over 2.7k Plasmids: Gateway® Cloning Vectors | More Plasmid Sets
No matches
| ||
Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C. |
| ||
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
| ||
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
| ||
Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA. |
| ||
Efficient cleavage requires at least two copies of the SacII recognition sequence. |
| ||
Sticky ends from different BspQI sites may not be compatible. |
| ||
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
|
| ||
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility. |
|
|
| ||
BsaHI is typically used at 37°C, but is even more active at 60°C. |
| ||
Efficient cleavage requires at least two copies of the NaeI recognition sequence. |
|
| ||
Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
|
|
| ||
BssHII is typically used at 50°C, but is 75% active at 37°C. |
|
|
| ||
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
| ||
SmaI can be used at 37°C for brief incubations. |
|
| ||
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. |
|