pHELLSGATE 12
High-throughput binary Gateway® vector with two introns, for silencing genes in plants using intron-containing hairpin RNA.
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BsiWI is typically used at 55°C, but is 50% active at 37°C. |
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FseI gradually loses activity when stored at -20°C. |
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Sticky ends from different Bsu36I sites may not be compatible. |
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Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT. |
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| * Blocked by Dcm methylation. Sticky ends from different SexAI sites may not be compatible. |
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| trfA 4407 .. 5555 = 1149 bp 382 amino acids = 43.8 kDa Product: trans-acting replication protein that binds to and activates oriV |
| trfA 4407 .. 5555 = 1149 bp 382 amino acids = 43.8 kDa Product: trans-acting replication protein that binds to and activates oriV |
| KanR 448 .. 1269 = 822 bp 273 amino acids = 29.9 kDa 3 segments Segment 1: nos 448 .. 489 = 42 bp 14 amino acids = 1.4 kDa Product: fusion between an N-terminal peptide of nopaline synthase and Tn5 aminoglycoside phosphotransferase confers resistance to kanamycin |
| KanR 448 .. 1269 = 822 bp 273 amino acids = 29.9 kDa 3 segments Segment 2: 490 .. 498 = 9 bp 3 amino acids = 443.5 Da Product: fusion between an N-terminal peptide of nopaline synthase and Tn5 aminoglycoside phosphotransferase confers resistance to kanamycin |
| KanR 448 .. 1269 = 822 bp 273 amino acids = 29.9 kDa 3 segments Segment 3: nptII 499 .. 1269 = 771 bp 256 amino acids = 28.1 kDa Product: fusion between an N-terminal peptide of nopaline synthase and Tn5 aminoglycoside phosphotransferase confers resistance to kanamycin |
| KanR 448 .. 1269 = 822 bp 273 amino acids = 29.9 kDa 3 segments Product: fusion between an N-terminal peptide of nopaline synthase and Tn5 aminoglycoside phosphotransferase confers resistance to kanamycin |
| SmR 8600 .. 9388 = 789 bp 262 amino acids = 29.2 kDa Product: aminoglycoside adenylyltransferase confers resistance to spectinomycin and streptomycin |
| SmR 8600 .. 9388 = 789 bp 262 amino acids = 29.2 kDa Product: aminoglycoside adenylyltransferase confers resistance to spectinomycin and streptomycin |
| IS1 3015 .. 3782 = 768 bp prokaryotic transposable element |
| IS1 3015 .. 3782 = 768 bp prokaryotic transposable element |
| PDK intron 14,475 .. 15,241 = 767 bp pyruvate orthophosphate dikinase intron from Flaveria trinervia |
| PDK intron 14,475 .. 15,241 = 767 bp pyruvate orthophosphate dikinase intron from Flaveria trinervia |