The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible.
PciI (5496) 1 site
ACATGTTGTACA
PciI is inhibited by nonionic detergents.
BspQI (5380) 1 site
GCTCTTCNCGAGAAGNNNN
Sticky ends from different BspQI sites may not be compatible.
SapI (5380) 1 site
GCTCTTCNCGAGAAGNNNN
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PpuMI (4588) 1 site
RGGWCCYYCCWGGR
Sticky ends from different PpuMI sites may not be compatible.