Sticky ends from different BspQI sites may not be compatible.
SapI (6266) 1 site
GCTCTTCNCGAGAAGNNNN
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
EagI (5431) 1 site
CGGCCGGCCGGC
BbsI (5400) 1 site
GAAGACNNCTTCTGNN(N)4
Sticky ends from different BbsI sites may not be compatible.BbsI gradually loses activity when stored at -20°C.