pcDNA3.1 nV5-DEST

Gateway® destination vector for expressing N-terminally V5-tagged proteins in mammalian cells.

Sequence Author: Thermo Fisher (Invitrogen)

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BglII (12) PvuI (6583) AhdI (6213) PciI (5323) PfoI (4747) BstBI (4654) RsrII (4488) PflFI - Tth111I (4090) PluTI (3975) SfoI (3973) NarI (3972) KasI (3971) AvrII (3762) StuI (3761) MfeI (161) NruI (208) SpeI (249) CMV enhancer NdeI (484) SnaBI (590) Eco53kI (816) SacI (818) HindIII (902) ATG TEV Site EcoO109I - PspOMI (973) ApaI (977) EcoRV (1003) NotI (1133) lac UV5 promoter BspEI (1449) BsmBI - Esp3I (1677) PasI - PflMI * (1685) BbvCI (2178) SrfI (2324) BmgBI (2358) BstXI (2441) BbsI (2925) DraIII (3239) CsiI - SexAI * (3529) pcDNA™3.1/nV5-DEST 7136 bp
BglII  (12)
1 site
A G A T C T T C T A G A
PvuI  (6583)
1 site
C G A T C G G C T A G C
AhdI  (6213)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (5323)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PfoI  (4747)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstBI  (4654)
1 site
T T C G A A A A G C T T
RsrII  (4488)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PflFI  (4090)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4090)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PluTI  (3975)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (3973)
1 site
G G C G C C C C G C G G
NarI  (3972)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (3971)
1 site
G G C G C C C C G C G G
AvrII  (3762)
1 site
C C T A G G G G A T C C
StuI  (3761)
1 site
A G G C C T T C C G G A
MfeI  (161)
1 site
C A A T T G G T T A A C
NruI  (208)
1 site
T C G C G A A G C