pcDNA6.2 C-YFP-DEST

Vivid Colors™ Gateway® destination vector for expressing C-terminally YFP-tagged proteins in mammalian cells.

Sequence Author: Thermo Fisher (Invitrogen)

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MfeI (31) SgrDI (7310) AhdI (6389) PciI (5496) BspQI - SapI (5380) PpuMI (4588) BbsI (4514) BclI * (4351) AvrII (4300) StuI (4299) CsiI - SexAI * (4067) MluI (98) SpeI (119) CMV enhancer NdeI (354) SnaBI (460) Eco53kI (686) SacI (688) NotI (911) lac UV5 promoter BspEI (1227) EcoRI (1231) BsmBI - Esp3I (1455) PflMI * (1463) BssHII (1725) BbvCI (1956) SrfI (2102) BmgBI (2136) BstXI (2219) BfuAI - BspMI (2346) PstI (2357) HpaI (2499) PmeI (3232) pcDNA™6.2/C-YFP-DEST 7442 bp
MfeI  (31)
1 site
C A A T T G G T T A A C
SgrDI  (7310)
1 site
C G T C G A C G G C A G C T G C
AhdI  (6389)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (5496)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (5380)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (5380)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PpuMI  (4588)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
BbsI  (4514)
1 site
G A A G A C N N