pcDNA6.2 C-YFP-DEST
Vivid Colors™ Gateway® destination vector for expressing C-terminally YFP-tagged proteins in mammalian cells.
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Sequence Author: Thermo Fisher (Invitrogen)
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The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
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PciI is inhibited by nonionic detergents. |
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Sticky ends from different BspQI sites may not be compatible. |
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Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
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Sticky ends from different PpuMI sites may not be compatible. |
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