pET161-DEST
Gateway® destination vector for high-level bacterial expression of proteins with a C-terminal tetracysteine (Lumio™) tag.
Sequence Author: Thermo Fisher (Invitrogen)
Explore Over 2.7k Plasmids: Gateway® Cloning Vectors | More Plasmid Sets
No matches
|
| ||
Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
|
| ||
The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
| ||
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
| ||
ApaI can be used between 25°C and 37°C. |
|
|
| ||
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
|
|
| ||
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible. |
| ||
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible. |
|
| ||
Sticky ends from different BspQI sites may not be compatible. |
| ||
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
| ||
PciI is inhibited by nonionic detergents. |
|
| ||
Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
|
|
|
|
| ||
Sticky ends from different PasI sites may not be compatible. |
|
|
|
| ||
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. |
| ||
SmaI can be used at 37°C for brief incubations. |
|
| ||
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. |
|
|
|
| ||
Sticky ends from different BlpI sites may not be compatible. |
|