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pLenti6.2 N-Lumio V5-DEST
Lentiviral Gateway® destination vector for N-terminal tagging of a protein with the Lumio™ tetracysteine tag.
Sequence Author: Thermo Fisher (Invitrogen)
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Plasmids
Gateway® Cloning Vectors
pLenti6.2 N-Lumio V5-DEST
Show Static Map
PciI
(8546)
AhdI
(7658)
DraIII
(6518)
NaeI
(6412)
NgoMIV
(6410)
AvrII
(6077)
SfiI
(6030)
SV40 poly(A) signal
PmlI
(4923)
PGK promoter
SpeI
(4658)
EcoNI
(942)
MfeI
(961)
BstAPI
(1225)
AleI
(1349)
KflI
(1706)
BspDI
-
ClaI
(1795)
NdeI
(2055)
SnaBI
(2161)
CMV promoter
tetracysteine tag
V5 tag
lac UV5 promoter
EcoRI
(3028)
PasI
-
PflMI
*
(3260)
BstZ17I
(3563)
TspMI
-
XmaI
(3897)
SmaI
-
SrfI
(3899)
BfuAI
-
BspMI
(4143)
PstI
(4154)
EcoRV
(4290)
PaeR7I
-
PspXI
-
XhoI
(4311)
PspOMI
(4323)
ApaI
(4327)
SacII
(4330)
BstBI
(4334)
MluI
(4378)
EcoNI
(942)
EcoNI
(942)
EcoNI
MfeI
(961)
MfeI
(961)
MfeI
BstAPI
(1225)
BstAPI
(1225)
BstAPI
AleI
(1349)
AleI
(1349)
AleI
KflI
(1706)
KflI
(1706)
KflI
ClaI
(1795)
ClaI
(1795)
ClaI
BspDI
-
(1795)
BspDI
-
(1795)
BspDI
-
NdeI
(2055)
NdeI
(2055)
NdeI
SnaBI
(2161)
SnaBI
(2161)
SnaBI
EcoRI
(3028)
EcoRI
(3028)
EcoRI
PasI
-
(3260)
PasI
-
(3260)
PasI
-
PflMI
*
(3260)
PflMI
*
(3260)
PflMI
*
BstZ17I
(3563)
BstZ17I
(3563)
BstZ17I
XmaI
(3897)
XmaI
(3897)
XmaI
TspMI
-
(3897)
TspMI
-
(3897)
TspMI
-
SmaI
-
(3899)
SmaI
-
(3899)
SmaI
-
SrfI
(3899)
SrfI
(3899)
SrfI
BfuAI
-
(4143)
BfuAI
-
(4143)
BfuAI
-
BspMI
(4143)
BspMI
(4143)
BspMI
PstI
(4154)
PstI
(4154)
PstI
EcoRV
(4290)
EcoRV
(4290)
EcoRV
PaeR7I
-
(4311)
PaeR7I
-
(4311)
PaeR7I
-
XhoI
(4311)
XhoI
(4311)
XhoI
PspXI
-
(4311)
PspXI
-
(4311)
PspXI
-
PspOMI
(4323)
PspOMI
(4323)
PspOMI
ApaI
(4327)
ApaI
(4327)
ApaI
SacII
(4330)
SacII
(4330)
SacII
BstBI
(4334)
BstBI
(4334)
BstBI
MluI
(4378)
MluI
(4378)
MluI
SpeI
(4658)
SpeI
(4658)
SpeI
PmlI
(4923)
PmlI
(4923)
PmlI
SfiI
(6030)
SfiI
(6030)
SfiI
AvrII
(6077)
AvrII
(6077)
AvrII
NgoMIV
(6410)
NgoMIV
(6410)
NgoMIV
NaeI
(6412)
NaeI
(6412)
NaeI
DraIII
(6518)
DraIII
(6518)
DraIII
AhdI
(7658)
AhdI
(7658)
AhdI
PciI
(8546)
PciI
(8546)
PciI
PGK promoter
PGK promoter
PGK promoter
PGK promoter
CMV promoter
CMV promoter
CMV promoter
CMV promoter
SV40 poly(A) signal
SV40 poly(A) signal
SV40 poly(A) signal
SV40 poly(A) signal
tetracysteine tag
tetracysteine tag
tetracysteine tag
tetracysteine tag
V5 tag
V5 tag
V5 tag
V5 tag
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
lac UV5 promoter
pLenti6.2/N-Lumio™/V5-DEST
8956 bp
PciI (8546)
1 site
A
C
A
T
G
T
T
G
T
A
C
A
PciI is inhibited by nonionic detergents.
AhdI (7658)
1 site
G
A
C
N
N
N
N
N
G
T
C
C
T
G
N
N
N
N
N
C
A
G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DraIII (6518)
1 site
C
A
C
N
N
N
G
T
G
G
T
G
N
N
N
C
A
C
Sticky ends from different DraIII sites may not be compatible.
NaeI (6412)
1 site
G
C
C
G
G
C
C
G
G
C
C
G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV (6410)
1 site
G
C
C
G
G
C
C
G
G
C
C
G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
AvrII (6077)
1 site
C
C
T
A
G
G
G
G
A
T
C
C
SfiI (6030)
1 site
G
G
C
C
N
N
N
N
N
G
G
C
C
C
C
G
G
N
N
N
N
N
C
C
G
G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PmlI (4923)
1 site
C
A
C
G
T
G
G
T
G
C
A
C
SpeI (4658)
1 site
A
C
T
A
G
T
T
G
A
T
C
A
EcoNI (942)
1 site
C
C
T
N
N
N
N
N
A
G
G
G
G
A
N
N
N
N