pLenti6.2 N-Lumio V5-DEST

Lentiviral Gateway® destination vector for N-terminal tagging of a protein with the Lumio™ tetracysteine tag.

Sequence Author: Thermo Fisher (Invitrogen)

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HomePlasmidsGateway® Cloning VectorspLenti6.2 N-Lumio V5-DEST
PciI (8546) AhdI (7658) DraIII (6518) NaeI (6412) NgoMIV (6410) AvrII (6077) SfiI (6030) SV40 poly(A) signal PmlI (4923) PGK promoter SpeI (4658) EcoNI (942) MfeI (961) BstAPI (1225) AleI (1349) KflI (1706) BspDI - ClaI (1795) NdeI (2055) SnaBI (2161) CMV promoter tetracysteine tag V5 tag lac UV5 promoter EcoRI (3028) PasI - PflMI * (3260) BstZ17I (3563) TspMI - XmaI (3897) SmaI - SrfI (3899) BfuAI - BspMI (4143) PstI (4154) EcoRV (4290) PaeR7I - PspXI - XhoI (4311) PspOMI (4323) ApaI (4327) SacII (4330) BstBI (4334) MluI (4378) pLenti6.2/N-Lumio™/V5-DEST 8956 bp
PciI  (8546)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
AhdI  (7658)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DraIII  (6518)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
NaeI  (6412)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (6410)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
AvrII  (6077)
1 site		 C C T A G G G G A T C C
SfiI  (6030)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PmlI  (4923)
1 site		 C A C G T G G T G C A C
SpeI  (4658)
1 site		 A C T A G T T G A T C A
EcoNI  (942)
1 site		 C C T N N N N N A G G G G A N N N N