pMCSG19B

Bacterial expression vector with an MBP-TVMV-6xHis-TEV leader and inducible TVMV protease. See also pMCSG19C.

Sequence Author: Midwest Center for Structural Genomics

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EagI - NotI (166) ScaI (6773) AhdI (6293) PciI (5400) BspQI - SapI (5284) BstZ17I (5171) PflFI - Tth111I (5145) Bpu10I (4506) PpuMI (4406) FspAI (4381) PshAI (4144) HpaI (3805) EcoRV (3749) SalI (179) Eco53kI (188) SacI (190) BamHI (198) SspI (219) Acc65I (244) KpnI (248) 6xHis TVMV site BsrGI (321) BsmI (824) BmgBI (900) BglII (1079) PsiI (1090) BsiWI (1148) RBS lac operator T7 promoter BspDI * - ClaI * (1549) SgrAI (1591) SphI (1747) SacII (2009) SpeI (2122) DraIII (2198) AgeI (2359) NsiI (2410) BseRI (2530) ZraI (2766) AatII (2768) SphI (2774) EcoNI (2834) PflMI (2881) BstEII (3480) PspOMI (3506) ApaI (3510) pMCSG19B 7468 bp