pMCSG62 (linearized)
Linearized bacterial vector for ligation-independent cloning (LIC), with a 6xHis-AviTag™-TEV leader plus the BirA biotin ligase plus tRNA genes for rare Arg and Ile codons.
Sequence Author: Midwest Center for Structural Genomics
Explore Over 2.7k Plasmids: Structural Genomics Vectors | More Plasmid Sets
No matches
|
|
|
| ||
Sticky ends from different BglI sites may not be compatible. |
| ||
Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
| ||
The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible. |
| ||
Sticky ends from different DrdI sites may not be compatible. |
| ||
PciI is inhibited by nonionic detergents. |
| ||
Sticky ends from different BspQI sites may not be compatible. |
| ||
Sticky ends from different SapI sites may not be compatible.SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot. |
|
| ||
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
| ||
Sticky ends from different BsmBI sites may not be compatible.BsmBI-v2 is an improved version of BsmBI. |
| ||
Sticky ends from different Esp3I sites may not be compatible. |
|
| ||
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
| ||
ApaI can be used between 25°C and 37°C. |
|
| ||
Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
| ||
Sticky ends from different BlpI sites may not be compatible. |
| ||
Sticky ends from different AvaI sites may not be compatible. |
| ||
Sticky ends from different BsoBI sites may not be compatible.BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C. |
| ||
PaeR7I does not recognize the sequence CTCTCGAG. |
|
|
|
|
|
|
|
|
|
| ||
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. |
| ||
Sticky ends from different BsmI sites may not be compatible. |
| ||
Sticky ends from different DraIII sites may not be compatible. |
|
|
|
|
| ||
Prolonged incubation with NdeI may lead to removal of additional nucleotides. |
|
| ||
| * Blocked by Dam methylation. |
| ||
| * Blocked by Dam methylation. |
|
|
|
| ||
Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
| ||
Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
|
|
|
|
| ||
The 1-base overhangs produced by EcoNI may be hard to ligate.Sticky ends from different EcoNI sites may not be compatible. |
| ||
Sticky ends from different PfoI sites may not be compatible. |
| ||
Sticky ends from different PflMI sites may not be compatible. |
|