pMCSG42

Bacterial vector encoding N-terminal MBP-TVMV and C-terminal TEV-6xHis plus TVMV protease. Identical to pMCSG34.

Sequence Author: Midwest Center for Structural Genomics

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6xHis ScaI (6750) AhdI (6270) PciI (5377) BspQI - SapI (5261) BstZ17I (5148) PflFI - Tth111I (5122) Bpu10I (4483) PpuMI (4383) FspAI (4358) PshAI (4121) HpaI (3782) EcoRV (3726) EagI - NotI (166) SalI (179) Eco53kI (188) SacI (190) BamHI (198) TEV site TspMI - XmaI (253) SmaI (255) BsrGI (298) BsmI (801) BmgBI (877) BglII (1056) PsiI (1067) BsiWI (1125) RBS T7 promoter BspDI * - ClaI * (1526) SgrAI (1568) ZraI (1728) AatII (1730) BseRI (1966) NsiI (2088) AgeI (2131) TVMV protease DraIII (2299) SpeI (2368) SacII (2487) EcoNI (2811) PflMI (2858) BstEII (3457) PspOMI (3483) ApaI (3487)