pMCSG42

Bacterial vector encoding N-terminal MBP-TVMV and C-terminal TEV-6xHis plus TVMV protease. Identical to pMCSG34.

Sequence Author: Midwest Center for Structural Genomics

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EagI - NotI (166) ScaI (6750) AhdI (6270) PciI (5377) BspQI - SapI (5261) BstZ17I (5148) PflFI - Tth111I (5122) Bpu10I (4483) PpuMI (4383) FspAI (4358) PshAI (4121) HpaI (3782) EcoRV (3726) SalI (179) Eco53kI (188) SacI (190) BamHI (198) 6xHis TEV site TspMI - XmaI (253) SmaI (255) BsrGI (298) BsmI (801) BmgBI (877) BglII (1056) PsiI (1067) BsiWI (1125) RBS lac operator T7 promoter BspDI * - ClaI * (1526) SgrAI (1568) ZraI (1728) AatII (1730) BseRI (1966) NsiI (2088) AgeI (2131) DraIII (2299) SpeI (2368) SacII (2487) EcoNI (2811) PflMI (2858) BstEII (3457) PspOMI (3483) ApaI (3487) pMCSG42 7445 bp