pMCSG35B

Bacterial vector encoding N-terminal 10xHis-MBP-TEV and C-terminal TEV-6xHis plus TVMV protease. See also pMCSG35.

Sequence Author: Midwest Center for Structural Genomics

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6xHis ScaI (6789) AhdI (6309) PciI (5416) BspQI - SapI (5300) BstZ17I (5187) PflFI - Tth111I (5161) Bpu10I (4522) PpuMI (4422) FspAI (4397) PshAI (4160) HpaI (3821) EagI - NotI (166) SalI (179) Eco53kI (188) SacI (190) BamHI (198) 6xHis TEV site TspMI - XmaI (253) SmaI (255) TEV site BsmI (786) BmgBI (862) PsiI (1052) BsiWI (1110) ATG RBS T7 promoter BspDI * - ClaI * (1565) SgrAI (1607) SphI (1763) SacII (2025) SpeI (2138) DraIII (2214) AgeI (2375) NsiI (2426) BseRI (2546) ZraI (2782) AatII (2784) SphI (2790) EcoNI (2850) PflMI (2897) BstEII (3496) PspOMI (3522) ApaI (3526)