pMCSG35B

Bacterial vector encoding N-terminal 10xHis-MBP-TEV and C-terminal TEV-6xHis plus TVMV protease. See also pMCSG35.

Sequence Author: Midwest Center for Structural Genomics

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HomePlasmidsStructural Genomics VectorspMCSG35B
EagI - NotI (166) ScaI (6789) AhdI (6309) PciI (5416) BspQI - SapI (5300) BstZ17I (5187) PflFI - Tth111I (5161) Bpu10I (4522) PpuMI (4422) FspAI (4397) PshAI (4160) HpaI (3821) SalI (179) Eco53kI (188) SacI (190) BamHI (198) 6xHis TEV site TspMI - XmaI (253) SmaI (255) BsmI (786) BmgBI (862) PsiI (1052) BsiWI (1110) 10xHis ATG RBS lac operator T7 promoter BspDI * - ClaI * (1565) SgrAI (1607) SphI (1763) SacII (2025) SpeI (2138) DraIII (2214) AgeI (2375) NsiI (2426) BseRI (2546) ZraI (2782) AatII (2784) SphI (2790) EcoNI (2850) PflMI (2897) BstEII (3496) PspOMI (3522) ApaI (3526) pMCSG35B 7484 bp