pMCSG35B (linearized)

Linearized bacterial vector for ligation-independent cloning (LIC), encoding N-terminal 10xHis-MBP-TEV and C-terminal TEV-6xHis plus TVMV protease. See also pMCSG35.

Sequence Author: Midwest Center for Structural Genomics

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HomePlasmidsStructural Genomics VectorspMCSG35B (linearized)
EagI - NotI (7395) ScaI (6534) AhdI (6054) PciI (5161) BspQI - SapI (5045) BstZ17I (4932) PflFI - Tth111I (4906) Bpu10I (4267) PpuMI (4167) FspAI (4142) PshAI (3905) HpaI (3566) ApaI (3271) SalI (7408) Eco53kI (7417) SacI (7419) BamHI (7427) TEV site End (7474) Start (0) BsmI (531) BmgBI (607) PsiI (797) BsiWI (855) 10xHis ATG RBS lac operator T7 promoter BspDI * - ClaI * (1310) SgrAI (1352) SphI (1508) SacII (1770) SpeI (1883) DraIII (1959) AgeI (2120) NsiI (2171) BseRI (2291) ZraI (2527) AatII (2529) SphI (2535) EcoNI (2595) PflMI (2642) BstEII (3241) PspOMI (3267) pMCSG35B 7474 bp