pMCSG35B (linearized)

Linearized bacterial vector for ligation-independent cloning (LIC), encoding N-terminal 10xHis-MBP-TEV and C-terminal TEV-6xHis plus TVMV protease. See also pMCSG35.

Sequence Author: Midwest Center for Structural Genomics

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EagI - NotI (7395) ScaI (6534) AhdI (6054) PciI (5161) BspQI - SapI (5045) BstZ17I (4932) PflFI - Tth111I (4906) Bpu10I (4267) PpuMI (4167) FspAI (4142) PshAI (3905) HpaI (3566) ApaI (3271) SalI (7408) Eco53kI (7417) SacI (7419) BamHI (7427) 6xHis End (7474) Start (0) BsmI (531) BmgBI (607) PsiI (797) BsiWI (855) 10xHis ATG RBS lac operator T7 promoter BspDI * - ClaI * (1310) SgrAI (1352) SphI (1508) SacII (1770) SpeI (1883) DraIII (1959) AgeI (2120) NsiI (2171) BseRI (2291) ZraI (2527) AatII (2529) SphI (2535) EcoNI (2595) PflMI (2642) BstEII (3241) PspOMI (3267)