pNYCOMPS-LIC-FH10T+ (N term)
Bacterial vector for high-throughput expression and purification of membrane proteins with N-terminal FLAG® and 10xHis tags.
Sequence Author: New York Structural Biology Center
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Sticky ends from different PasI sites may not be compatible. |
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BsrGI is typically used at 37°C, but is even more active at 60°C. |
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This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. |
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Sticky ends from different BsaI sites may not be compatible.BsaI can be used between 37°C and 50°C. |
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Efficient cleavage requires at least two copies of the BfuAI recognition sequence. Sticky ends from different BfuAI sites may not be compatible.BfuAI is typically used at 50°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the BspMI recognition sequence. Sticky ends from different BspMI sites may not be compatible. |
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Efficient cleavage requires at least two copies of the SgrAI recognition sequence. |
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Sticky ends from different BstAPI sites may not be compatible. |
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* Blocked by Dam methylation. BclI is typically used at 50-55°C, but is 50% active at 37°C. |
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Sticky ends from different BstEII sites may not be compatible.BstEII is typically used at 60°C, but is 50% active at 37°C. |
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Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be compatible.For full activity, add fresh S-adenosylmethionine (SAM). |
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ApaI can be used between 25°C and 37°C. |
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EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. |
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PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations. |
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Sticky ends from different BglI sites may not be compatible. |
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PaeR7I does not recognize the sequence CTCTCGAG. |
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