pMCSG34

Bacterial vector encoding N-terminal MBP-TVMV and C-terminal TEV-6xHis plus TVMV protease. See also pMCSG34B.

Sequence Author: Midwest Center for Structural Genomics

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6xHis ScaI (6750) AhdI (6270) PciI (5377) BspQI - SapI (5261) BstZ17I (5148) PflFI - Tth111I (5122) Bpu10I (4483) PpuMI (4383) FspAI (4358) PshAI (4121) HpaI (3782) EcoRV (3726) EagI - NotI (166) SalI (179) Eco53kI (188) SacI (190) BamHI (198) TEV site TspMI - XmaI (253) SmaI (255) BsrGI (298) BsmI (801) BmgBI (877) BglII (1056) PsiI (1067) BsiWI (1125) RBS T7 promoter BspDI * - ClaI * (1526) SgrAI (1568) SphI (1724) ZraI (1728) AatII (1730) BseRI (1966) NsiI (2088) AgeI (2131) TVMV protease DraIII (2299) SpeI (2368) SacII (2487) SphI (2751) EcoNI (2811) PflMI (2858) BstEII (3457) PspOMI (3483) ApaI (3487)