pVP33K

Bacterial Flexi® Vector with a kanamycin resistance marker, for appending an N-terminal 8xHis-MBP-HRV 3C-tetracysteine-TEV cassette to an expressed protein.

Sequence Author: Center for Eukaryotic Structural Genomics

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EagI (7040) NaeI (6448) NgoMIV (6446) RsrII (6431) AvrII (6279) PciI (5459) NdeI (5282) BstZ17I (5232) FspAI (5039) PpuMI * (5022) BstEII (4387) EcoRV (4123) HpaI (4067) PaeR7I - XhoI (117) AanI (165) MfeI (175) lac operator RBS BseRI (231) ATG NsiI (263) BsaI (327) AanI (616) BmgBI (806) AfeI (1174) PacI (1369) tetracysteine tag TEV site AsiSI - SgfI (1438) PmeI (2564) Eco53kI (2577) SacI (2579) SbfI (2612) BsrGI (2721) AscI (2738) SgrDI (2753) BspDI - ClaI (2766) BstBI (2777) HindIII (2780) NheI (2900) BmtI (2904)