pMCSG34 (linearized)

Linearized bacterial vector for ligation-independent cloning (LIC), encoding N-terminal MBP-TVMV and C-terminal TEV-6xHis plus TVMV protease. See also pMCSG34B.

Sequence Author: Midwest Center for Structural Genomics

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HomePlasmidsStructural Genomics VectorspMCSG34 (linearized)
EagI - NotI (7356) ScaI (6495) AhdI (6015) PciI (5122) BspQI - SapI (5006) BstZ17I (4893) PflFI - Tth111I (4867) Bpu10I (4228) PpuMI (4128) FspAI (4103) PshAI (3866) HpaI (3527) EcoRV (3471) ApaI (3232) SalI (7369) Eco53kI (7378) SacI (7380) BamHI (7388) TEV site End (7435) Start (0) BsrGI (43) BsmI (546) BmgBI (622) BglII (801) PsiI (812) BsiWI (870) RBS T7 promoter BspDI * - ClaI * (1271) SgrAI (1313) SphI (1469) ZraI (1473) AatII (1475) BseRI (1711) NsiI (1833) AgeI (1876) DraIII (2044) SpeI (2113) SacII (2232) SphI (2496) EcoNI (2556) PflMI (2603) BstEII (3202) PspOMI (3228)