pMCSG41

Bacterial vector encoding a C-terminal TEV-6xHis cassette plus TVMV protease, for high-throughput purification of recombinant proteins.

Sequence Author: Midwest Center for Structural Genomics

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HomePlasmidsStructural Genomics VectorspMCSG41
T7 terminator ScaI (5585) PvuI (5475) BsaI (5166) AhdI (5105) PciI (4212) BspQI - SapI (4096) BstZ17I (3983) BsaAI (3964) PflFI - Tth111I (3957) Bpu10I (3318) PpuMI (3218) FspAI (3193) BlpI (80) 6xHis EagI - NotI (166) SalI (179) Eco53kI (188) SacI (190) BamHI (198) 6xHis TEV site TspMI - XmaI (253) SmaI (255) RBS lac operator T7 promoter BspDI * - ClaI * (361) SgrAI (403) SphI (559) ZraI (563) AatII (565) NdeI (737) BseRI (801) NsiI (923) AgeI (966) DraIII (1134) SpeI (1203) SacII (1322) NcoI (1458) SphI (1586) EcoNI (1646) PflMI (1693) BstAPI (1794) BclI * (2125) BstEII (2292) PspOMI (2318) ApaI (2322) BssHII (2522) EcoRV (2561) HpaI (2617) PshAI (2956) pMCSG41 6280 bp
ScaI  (5585)
1 site		 A G T A C T T C A T G A
PvuI  (5475)
1 site		 C G A T C G G C T A G C
BsaI  (5166)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (5105)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (4212)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BspQI  (4096)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (4096)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstZ17I  (3983)
1 site		 G T A T A C C A T A T G
BsaAI  (3964)
1 site		 Y A C G T R R T G C A Y
PflFI  (3957)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3957)
1 site		 G A C N N