pMCSG41

Bacterial vector encoding a C-terminal TEV-6xHis cassette plus TVMV protease, for high-throughput purification of recombinant proteins.

Sequence Author: Midwest Center for Structural Genomics

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ScaI (5585) PvuI (5475) BsaI (5166) AhdI (5105) PciI (4212) BspQI - SapI (4096) BstZ17I (3983) BsaAI (3964) PflFI - Tth111I (3957) Bpu10I (3318) PpuMI (3218) FspAI (3193) BlpI (80) 6xHis EagI - NotI (166) SalI (179) Eco53kI (188) SacI (190) BamHI (198) 6xHis TEV site TspMI - XmaI (253) SmaI (255) RBS T7 promoter BspDI * - ClaI * (361) SgrAI (403) SphI (559) ZraI (563) AatII (565) NdeI (737) BseRI (801) NsiI (923) AgeI (966) DraIII (1134) SpeI (1203) SacII (1322) NcoI (1458) SphI (1586) EcoNI (1646) PflMI (1693) BstAPI (1794) BclI * (2125) BstEII (2292) PspOMI (2318) ApaI (2322) BssHII (2522) EcoRV (2561) HpaI (2617) PshAI (2956) pMCSG41 6280 bp