pMCSG41

Bacterial vector encoding a C-terminal TEV-6xHis cassette plus TVMV protease, for high-throughput purification of recombinant proteins.

Sequence Author: Midwest Center for Structural Genomics

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T7 terminator ScaI (5585) PvuI (5475) BsaI (5166) AhdI (5105) PciI (4212) BspQI - SapI (4096) BstZ17I (3983) BsaAI (3964) PflFI - Tth111I (3957) Bpu10I (3318) PpuMI (3218) FspAI (3193) BlpI (80) 6xHis EagI - NotI (166) SalI (179) Eco53kI (188) SacI (190) BamHI (198) 6xHis TEV site TspMI - XmaI (253) SmaI (255) RBS lac operator T7 promoter BspDI * - ClaI * (361) SgrAI (403) SphI (559) ZraI (563) AatII (565) NdeI (737) BseRI (801) NsiI (923) AgeI (966) DraIII (1134) SpeI (1203) SacII (1322) NcoI (1458) SphI (1586) EcoNI (1646) PflMI (1693) BstAPI (1794) BclI * (2125) BstEII (2292) PspOMI (2318) ApaI (2322) BssHII (2522) EcoRV (2561) HpaI (2617) PshAI (2956) pMCSG41 6280 bp
ScaI  (5585)
1 site
A G T A C T T C A T G A
PvuI  (5475)
1 site
C G A T C G G C T A G C
BsaI  (5166)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (5105)
1 site
G A C N N N N N G T C C T G N N N