pMCSG35 (linearized)

Linearized bacterial vector for ligation-independent cloning (LIC), encoding N-terminal 10xHis-MBP-TEV and C-terminal TEV-6xHis plus TVMV protease. See also pMCSG35B.

Sequence Author: Midwest Center for Structural Genomics

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HomePlasmidsStructural Genomics VectorspMCSG35 (linearized)
EagI - NotI (7395) ScaI (6534) AhdI (6054) PciI (5161) BspQI - SapI (5045) BstZ17I (4932) PflFI - Tth111I (4906) Bpu10I (4267) PpuMI (4167) FspAI (4142) PshAI (3905) HpaI (3566) ApaI (3271) SalI (7408) Eco53kI (7417) SacI (7419) BamHI (7427) TEV site End (7474) Start (0) BsmI (531) BmgBI (607) PsiI (797) BsiWI (855) ATG RBS T7 promoter BspDI * - ClaI * (1310) SgrAI (1352) SphI (1508) ZraI (1512) AatII (1514) BseRI (1750) NsiI (1872) AgeI (1915) TVMV protease DraIII (2083) SpeI (2152) SacII (2271) SphI (2535) EcoNI (2595) PflMI (2642) BstEII (3241) PspOMI (3267)