pMCSG38 (linearized)

Linearized bacterial vector for ligation-independent cloning (LIC), with a 6xHis-TEV leader and a Tet-inducible TVMV protease cassette. See also pMCSG38C.

Sequence Author: Midwest Center for Structural Genomics

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T7 terminator ScaI (5399) PvuI (5289) BsaI (4980) AhdI (4919) PciI (4026) BspQI - SapI (3910) BstZ17I (3797) BsaAI (3778) PflFI - Tth111I (3771) Bpu10I (3132) PpuMI (3032) FspAI (3007) BlpI (6174) EagI - NotI (6260) SalI (6273) Eco53kI (6282) SacI (6284) BamHI (6292) End (6298) Start (0) TEV site Acc65I (25) KpnI (29) BglII (32) 6xHis ATG RBS lac operator T7 promoter BspDI * - ClaI * (175) SgrAI (217) SphI (373) NcoI (493) SacII (635) SpeI (748) DraIII (824) AgeI (985) NsiI (1036) BseRI (1156) ZraI (1392) AatII (1394) SphI (1400) EcoNI (1460) PflMI (1507) BstAPI (1608) BclI * (1939) BstEII (2106) PspOMI (2132) ApaI (2136) BssHII (2336) EcoRV (2375) HpaI (2431) PshAI (2770) pMCSG38 6298 bp
ScaI  (5399)
1 site
A G T A C T T C A T G A
PvuI  (5289)
1 site
C G A T C G G C T A G C
BsaI  (4980)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (4919)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (4026)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (3910)
1 site
G