pMCSG19C

Bacterial expression vector with an MBP-TVMV-6xHis-TEV leader and inducible TVMV protease. See also pMCSG19B.

Sequence Author: Midwest Center for Structural Genomics

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6xHis ScaI (6773) AhdI (6293) PciI (5400) BspQI - SapI (5284) BstZ17I (5171) PflFI - Tth111I (5145) Bpu10I (4506) PpuMI (4406) FspAI (4381) PshAI (4144) HpaI (3805) EcoRV (3749) EagI - NotI (166) SalI (179) Eco53kI (188) SacI (190) BamHI (198) SspI (219) TEV site Acc65I (244) KpnI (248) 6xHis TVMV site BsrGI (321) BsmI (824) BmgBI (900) BglII (1079) PsiI (1090) BsiWI (1148) RBS lac operator T7 promoter BspDI * - ClaI * (1549) SgrAI (1591) SphI (1747) ZraI (1751) AatII (1753) BseRI (1989) NsiI (2111) AgeI (2154) DraIII (2322) SpeI (2391) SacII (2510) SphI (2774) EcoNI (2834) PflMI (2881) BstEII (3480) PspOMI (3506) ApaI (3510) pMCSG19C 7468 bp