pMCSG19C (linearized)

Linearized bacterial expression vector for ligation-independent cloning (LIC), with an MBP-TVMV-6xHis-TEV leader  and inducible TVMV protease. See also pMCSG19B.

Sequence Author: Midwest Center for Structural Genomics

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6xHis ScaI (6554) AhdI (6074) PciI (5181) BspQI - SapI (5065) BstZ17I (4952) PflFI - Tth111I (4926) Bpu10I (4287) PpuMI (4187) FspAI (4162) PshAI (3925) HpaI (3586) EcoRV (3530) ApaI (3291) EagI - NotI (7415) SalI (7428) Eco53kI (7437) SacI (7439) BamHI (7447) End (7453) Start (0) Acc65I (25) KpnI (29) 6xHis BsrGI (102) BsmI (605) BmgBI (681) BglII (860) PsiI (871) BsiWI (929) RBS lac operator T7 promoter BspDI * - ClaI * (1330) SgrAI (1372) SphI (1528) ZraI (1532) AatII (1534) BseRI (1770) NsiI (1892) AgeI (1935) DraIII (2103) SpeI (2172) SacII (2291) SphI (2555) EcoNI (2615) PflMI (2662) BstEII (3261) PspOMI (3287)