pMCSG42 (linearized)

Linearized bacterial vector for ligation-independent cloning (LIC), encoding N-terminal MBP-TVMV and C-terminal TEV-6xHis plus TVMV protease. Identical to pMCSG34.

Sequence Author: Midwest Center for Structural Genomics

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EagI - NotI (7356) ScaI (6495) AhdI (6015) PciI (5122) BspQI - SapI (5006) BstZ17I (4893) PflFI - Tth111I (4867) Bpu10I (4228) PpuMI (4128) FspAI (4103) PshAI (3866) HpaI (3527) EcoRV (3471) SalI (7369) Eco53kI (7378) SacI (7380) BamHI (7388) 6xHis End (7435) Start (0) BsrGI (43) BsmI (546) BmgBI (622) BglII (801) PsiI (812) BsiWI (870) RBS lac operator T7 promoter BspDI * - ClaI * (1271) SgrAI (1313) SphI (1469) ZraI (1473) AatII (1475) BseRI (1711) NsiI (1833) AgeI (1876) DraIII (2044) SpeI (2113) SacII (2232) SphI (2496) EcoNI (2556) PflMI (2603) BstEII (3202) PspOMI (3228) ApaI (3232)