pMCSG35

Bacterial vector encoding N-terminal 10xHis-MBP-TEV and C-terminal TEV-6xHis plus TVMV protease. See also pMCSG35B.

Sequence Author: Midwest Center for Structural Genomics

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HomePlasmidsStructural Genomics VectorspMCSG35
EagI - NotI (166) ScaI (6789) AhdI (6309) PciI (5416) BspQI - SapI (5300) BstZ17I (5187) PflFI - Tth111I (5161) Bpu10I (4522) PpuMI (4422) FspAI (4397) PshAI (4160) HpaI (3821) SalI (179) Eco53kI (188) SacI (190) BamHI (198) 6xHis TEV site TspMI - XmaI (253) SmaI (255) BsmI (786) BmgBI (862) PsiI (1052) BsiWI (1110) ATG RBS T7 promoter BspDI * - ClaI * (1565) SgrAI (1607) SphI (1763) ZraI (1767) AatII (1769) BseRI (2005) NsiI (2127) AgeI (2170) DraIII (2338) SpeI (2407) SacII (2526) SphI (2790) EcoNI (2850) PflMI (2897) BstEII (3496) PspOMI (3522) ApaI (3526)