pMCSG35

Bacterial vector encoding N-terminal 10xHis-MBP-TEV and C-terminal TEV-6xHis plus TVMV protease. See also pMCSG35B.

Sequence Author: Midwest Center for Structural Genomics

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HomePlasmidsStructural Genomics VectorspMCSG35
6xHis ScaI (6789) AhdI (6309) PciI (5416) BspQI - SapI (5300) BstZ17I (5187) PflFI - Tth111I (5161) Bpu10I (4522) PpuMI (4422) FspAI (4397) PshAI (4160) HpaI (3821) EagI - NotI (166) SalI (179) Eco53kI (188) SacI (190) BamHI (198) 6xHis TEV site TspMI - XmaI (253) SmaI (255) TEV site BsmI (786) BmgBI (862) PsiI (1052) BsiWI (1110) 10xHis ATG RBS lac operator T7 promoter BspDI * - ClaI * (1565) SgrAI (1607) SphI (1763) ZraI (1767) AatII (1769) BseRI (2005) NsiI (2127) AgeI (2170) DraIII (2338) SpeI (2407) SacII (2526) SphI (2790) EcoNI (2850) PflMI (2897) BstEII (3496) PspOMI (3522) ApaI (3526)