pVP91A

Bacterial Flexi® Vector with an ampicillin resistance marker, for appending an N-terminal 8xHis tag to an expressed protein.

Sequence Author: Center for Eukaryotic Structural Genomics

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EcoO109I (100) BsiWI (6529) FspI (5887) BglI (5785) BsaI (5726) AhdI (5665) AvrII (5587) AlwNI (5183) BspQI - SapI (4651) NdeI (4590) PflFI - Tth111I (4514) PfoI (4411) BclI * (3902) BstEII (3734) ApaI (3713) PspOMI (3709) PluTI (3281) SfoI (3279) NarI * (3278) PaeR7I - XhoI (117) AanI (165) MfeI (175) lac operator RBS BseRI (231) ATG NsiI (235) 8xHis AsiSI - SgfI (262) StuI * (744) AanI (1143) SnaBI (1252) BtgI (1579) SacII (1582) BspEI (2303) PasI (2539) PmeI (2758) Eco53kI (2771) SacI (2773) TspMI - XmaI (2779) SmaI (2781) PstI - SbfI (2806) BfuAI - BspMI (2809) SphI (2812) BlpI (2871) AscI (2929) SgrDI (2944) BstBI (2968) KasI (3277) pVP91A 6534 bp
EcoO109I  (100)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsiWI  (6529)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
FspI  (5887)
1 site
T G C G C A A C G C G T
BglI  (5785)
1 site
G C C N N N N N G G C