pVP56A

Bacterial Flexi® Vector with an ampicillin resistance marker, for appending an N-terminal 8xHis-MBP-HRV 3C-TEV cassette to an expressed protein.

Sequence Author: Center for Eukaryotic Structural Genomics

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PaeR7I - XhoI (37) XmnI (6865) BglI (6386) AhdI (6266) PciI (5373) BspQI - SapI (5257) NdeI (5196) BstZ17I (5146) PflFI - Tth111I (5120) FspAI (4953) PpuMI * (4936) BstEII (4301) EcoRV (4037) HpaI (3981) PluTI (3848) SfoI (3846) NarI * (3845) T5 promoter AanI (85) MfeI (95) lac operator RBS BseRI (151) ATG NsiI (183) BsiWI (474) AanI (536) BglII (543) BmgBI (726) BsaBI (859) AfeI (1094) PacI (1289) TEV site AsiSI - SgfI (1355) PmeI (2481) Eco53kI (2494) SacI (2496) PstI - SbfI (2529) SphI (2535) BsrGI (2635) AscI (2652) SgrDI (2667) BspDI - ClaI (2680) BstBI (2691) HindIII (2694) NheI (2812) BmtI (2816) KasI (3844) pVP56A 7222 bp