pVP56A

Bacterial Flexi® Vector with an ampicillin resistance marker, for appending an N-terminal 8xHis-MBP-HRV 3C-TEV cassette to an expressed protein.

Sequence Author: Center for Eukaryotic Structural Genomics

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PaeR7I - XhoI (37) XmnI (6865) BglI (6386) AhdI (6266) PciI (5373) BspQI - SapI (5257) NdeI (5196) BstZ17I (5146) PflFI - Tth111I (5120) FspAI (4953) PpuMI * (4936) BstEII (4301) EcoRV (4037) HpaI (3981) PluTI (3848) SfoI (3846) NarI * (3845) KasI (3844) T5 promoter AanI (85) MfeI (95) lac operator RBS BseRI (151) ATG 8xHis NsiI (183) BsiWI (474) AanI (536) BglII (543) BmgBI (726) BsaBI (859) AfeI (1094) PacI (1289) TEV site AsiSI - SgfI (1355) lac UV5 promoter PmeI (2481) Eco53kI (2494) SacI (2496) PstI - SbfI (2529) SphI (2535) BsrGI (2635) AscI (2652) SgrDI (2667) BspDI - ClaI (2680) BstBI (2691) HindIII (2694) NheI (2812) BmtI (2816) pVP56A 7222 bp
PaeR7I  (37)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (37)
1 site
C T C G A G G A G C T C
XmnI  (6865)
1 site
G A A N N N N T T C C T T N N N N A A G
BglI  (6386)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
AhdI  (6266)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PciI  (5373)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (5257)
1 site
G C T C T T C