pMCSG41 (linearized)

Linearized bacterial vector for ligation-independent cloning (LIC), with a C-terminal TEV-6xHis cassette plus TVMV protease.

Sequence Author: Midwest Center for Structural Genomics

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T7 terminator ScaI (5330) PvuI (5220) BsaI (4911) AhdI (4850) PciI (3957) BspQI - SapI (3841) BstZ17I (3728) BsaAI (3709) PflFI - Tth111I (3702) Bpu10I (3063) PpuMI (2963) FspAI (2938) BlpI (6105) 6xHis EagI - NotI (6191) SalI (6204) Eco53kI (6213) SacI (6215) BamHI (6223) 6xHis End (6270) Start (0) RBS lac operator T7 promoter BspDI * - ClaI * (106) SgrAI (148) SphI (304) ZraI (308) AatII (310) NdeI (482) BseRI (546) NsiI (668) AgeI (711) DraIII (879) SpeI (948) SacII (1067) NcoI (1203) SphI (1331) EcoNI (1391) PflMI (1438) BstAPI (1539) BclI * (1870) BstEII (2037) PspOMI (2063) ApaI (2067) BssHII (2267) EcoRV (2306) HpaI (2362) PshAI (2701) pMCSG41 6270 bp
ScaI  (5330)
1 site
A G T A C T T C A T G A
PvuI  (5220)
1 site
C G A T C G G C T A G C
BsaI  (4911)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (4850)
1 site