pMCSG41 (linearized)

Linearized bacterial vector for ligation-independent cloning (LIC), with a C-terminal TEV-6xHis cassette plus TVMV protease.

Sequence Author: Midwest Center for Structural Genomics

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BlpI (6105) ScaI (5330) PvuI (5220) BsaI (4911) AhdI (4850) PciI (3957) BspQI - SapI (3841) BstZ17I (3728) BsaAI (3709) PflFI - Tth111I (3702) Bpu10I (3063) PpuMI (2963) FspAI (2938) 6xHis EagI - NotI (6191) SalI (6204) Eco53kI (6213) SacI (6215) BamHI (6223) TEV site End (6270) Start (0) RBS lac operator T7 promoter BspDI * - ClaI * (106) SgrAI (148) SphI (304) ZraI (308) AatII (310) NdeI (482) BseRI (546) NsiI (668) AgeI (711) DraIII (879) SpeI (948) SacII (1067) NcoI (1203) SphI (1331) EcoNI (1391) PflMI (1438) BstAPI (1539) BclI * (1870) BstEII (2037) PspOMI (2063) ApaI (2067) BssHII (2267) EcoRV (2306) HpaI (2362) PshAI (2701) pMCSG41 6270 bp