pMCSG19B (linearized)

Linearized bacterial expression vector for ligation-independent cloning (LIC), with an MBP-TVMV-6xHis-TEV leader and inducible TVMV protease. See also pMCSG19C.

Sequence Author: Midwest Center for Structural Genomics

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HomePlasmidsStructural Genomics VectorspMCSG19B (linearized)
6xHis ScaI (6554) AhdI (6074) PciI (5181) BspQI - SapI (5065) BstZ17I (4952) PflFI - Tth111I (4926) Bpu10I (4287) PpuMI (4187) FspAI (4162) PshAI (3925) HpaI (3586) EcoRV (3530) ApaI (3291) EagI - NotI (7415) SalI (7428) Eco53kI (7437) SacI (7439) BamHI (7447) End (7453) Start (0) Acc65I (25) KpnI (29) 6xHis TVMV site BsrGI (102) BsmI (605) BmgBI (681) BglII (860) PsiI (871) BsiWI (929) RBS lac operator T7 promoter BspDI * - ClaI * (1330) SgrAI (1372) SphI (1528) SacII (1790) SpeI (1903) DraIII (1979) AgeI (2140) NsiI (2191) BseRI (2311) ZraI (2547) AatII (2549) SphI (2555) EcoNI (2615) PflMI (2662) BstEII (3261) PspOMI (3287) pMCSG19B 7453 bp