pVP33A

Bacterial Flexi® Vector with an ampicillin resistance marker, for appending an N-terminal 8xHis-MBP-HRV 3C-tetracysteine-TEV cassette to an expressed protein.

Sequence Author: Center for Eukaryotic Structural Genomics

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PaeR7I - XhoI (37) XmnI (6871) BglI (6392) AhdI (6272) PciI (5379) BspQI - SapI (5263) NdeI (5202) BstZ17I (5152) PflFI - Tth111I (5126) FspAI (4959) PpuMI * (4942) BstEII (4307) EcoRV (4043) HpaI (3987) PluTI (3854) SfoI (3852) NarI * (3851) KasI (3850) T5 promoter AanI (85) MfeI (95) lac operator RBS BseRI (151) ATG 8xHis NsiI (183) BsiWI (474) AanI (536) BglII (543) BmgBI (726) BsaBI (859) AfeI (1094) PacI (1289) tetracysteine tag TEV site AsiSI - SgfI (1358) lac UV5 promoter PmeI (2484) Eco53kI (2497) SacI (2499) PstI - SbfI (2532) SphI (2538) BsrGI (2641) AscI (2658) SgrDI (2673) BspDI - ClaI (2686) BstBI (2697) HindIII (2700) NheI (2818) BmtI (2822) pVP33A 7228 bp
PaeR7I  (37)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.