pMCSG38C (linearized)

Linearized bacterial vector for ligation-independent cloning (LIC), with a 6xHis-TEV leader and a Tet-inducible TVMV protease cassette. See also pMCSG38.

Sequence Author: Midwest Center for Structural Genomics

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HomePlasmidsStructural Genomics VectorspMCSG38C (linearized)
T7 terminator ScaI (5399) PvuI (5289) BsaI (4980) AhdI (4919) PciI (4026) BspQI - SapI (3910) BstZ17I (3797) BsaAI (3778) PflFI - Tth111I (3771) Bpu10I (3132) PpuMI (3032) FspAI (3007) BlpI (6174) EagI - NotI (6260) SalI (6273) Eco53kI (6282) SacI (6284) BamHI (6292) End (6298) Start (0) TEV site Acc65I (25) KpnI (29) BglII (32) 6xHis ATG RBS lac operator T7 promoter BspDI * - ClaI * (175) SgrAI (217) SphI (373) ZraI (377) AatII (379) BseRI (615) NsiI (737) AgeI (780) DraIII (948) SpeI (1017) SacII (1136) NcoI (1272) SphI (1400) EcoNI (1460) PflMI (1507) BstAPI (1608) BclI * (1939) BstEII (2106) PspOMI (2132) ApaI (2136) BssHII (2336) EcoRV (2375) HpaI (2431) PshAI (2770) pMCSG38C 6298 bp