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Gateway® Cloning Vectors
Commonly used Gateway® sequences including Donor Vectors, Entry Vectors, and Destination Vectors
Updated September 14th, 2021
These combined DNA sequence and map files can be opened with 
SnapGene or the free 
SnapGene Viewer.
Gateway Cloning Vectors.zip (3 MB)
106 Sequences
106 Sequences
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Gateway® Cloning Vectors
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Individual Sequences & Maps
- pAd/BLOCK-iT-DEST
- pAd/CMV/V5-DEST
- pAd/PL-DEST
- pBAD-DEST49
- pcDNA-DEST40
- pcDNA-DEST47
- pcDNA-DEST53
- pcDNA3.1/nV5-DEST
- pcDNA3.2/capTEV-CT/V5-DEST
- pcDNA3.2/capTEV-NT/V5-DEST
- pcDNA3.2/V5-DEST
- pcDNA6.2/C-EmGFP-DEST
- pcDNA6.2/C-YFP-DEST
- pcDNA6.2/cGeneBLAzer-DEST
- pcDNA6.2/cLumio-DEST
- pcDNA6.2/EmGFP-Bsd/V5-DEST
- pcDNA6.2/N-EmGFP-DEST
- pcDNA6.2/N-YFP-DEST
- pcDNA6.2/nGeneBLAzer-DEST
- pcDNA6.2/nLumio-DEST
- pcDNA6.2/V5-DEST
- pcDNA6.2/V5-pL-DEST
- pCMV SPORT6
- pCMV SPORT6.1
- pCOLA-2-DEST
- pCOLA-3-DEST
- pDEST8
- pDEST10
- pDEST14
- pDEST15
- pDEST17
- pDEST20
- pDEST24
- pDEST26
- pDEST27
- pDEST R4-R3
- pDEST R4-R3 Vector II
- pDONR201
- pDONR207
- pDONR221
- pDONR221 P1-P4
- pDONR221 P1-P5r
- pDONR221 P3-P2
- pDONR221 P4r-P3r
- pDONR221 P5-P2
- pDONR221 P5-P4
- pDONR222
- pDONR223
- pDONR P2r-P3
- pDONR P4-P1r
- pDONR/Zeo
- pEF-DEST51
- pEF5/FRT/V5-DEST
- pENTR1A
- pENTR2B
- pENTR3C
- pENTR4
- pENTR11
- pENTR/GeneBLAzer
- pET-53-DEST
- pET-54-DEST
- pET-55-DEST
- pET-56-DEST
- pET-57-DEST
- pET-58-DEST
- pET-59-DEST
- pET-60-DEST
- pET-61-DEST
- pET-62-DEST
- pET-DEST42
- pET104-DEST
- pET104.1-DEST
- pET160-DEST
- pET161-DEST
- pEXP1-DEST
- pEXP2-DEST
- pEXP3-DEST
- pEXP4-DEST
- pHELLSGATE
- pHELLSGATE 4
- pHELLSGATE 8
- pHELLSGATE 12
- pIB/V5-His-DEST
- pLenti4/BLOCK-iT-DEST
- pLenti4/V5-DEST
- pLenti6.2/C-Lumio/V5-DEST
- pLenti6.2/N-Lumio/V5-DEST
- pLenti6.2/V5-DEST
- pLenti6.3/TO/V5-DEST
- pLenti6.3/V5-DEST
- pLenti6.4/R4R2/V5-DEST
- pLenti6/BLOCK-iT-DEST
- pLenti6/capTEV-CT-DEST
- pLenti6/capTEV-NT-DEST1
- pLenti6/UbC/V5-DEST
- pLenti6/V5-DEST
- pLenti7.3/V5-DEST
- pMT-DEST48
- pMT/BioEase-DEST
- pSCREEN-iT/lacZ-DEST
- pT-REx-DEST30
- pT-REx-DEST31
- pYES-DEST52
- Reading Frame Cassette A
- Reading Frame Cassette B
- Reading Frame Cassette C.1
The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.
