Gateway® Cloning Vectors

Commonly used Gateway® sequences including Donor Vectors, Entry Vectors, and Destination Vectors

Download Plasmid Set Try SnapGene for Free
Download SnapGene Viewer
Explore Over 2.7k Plasmids: More Plasmid Sets
HomeResourcesPlasmid FilesGateway® Cloning Vectors

Individual Sequences & Maps

Reading Frame Cassette A
pDONR221 P3-P2
pET-DEST42
pLenti7.3 V5-DEST
Reading Frame Cassette B
pDONR221 P4r-P3r
pET104-DEST
pMT-DEST48
Reading Frame Cassette C.1
pDONR221 P5-P2
pET104.1-DEST
pMT BioEase-DEST
pAd BLOCK-iT-DEST
pDONR221 P5-P4
pET160-DEST
pSCREEN-iT lacZ-DEST
pAd CMV V5-DEST
pDONR222
pET161-DEST
pT-REx-DEST30
pAd PL-DEST
pDONR223
pEXP1-DEST
pT-REx-DEST31
pBAD-DEST49
pDONR P2r-P3
pEXP2-DEST
pYES-DEST52
pCMV SPORT6
pDONR P4-P1r
pEXP3-DEST
pcDNA-DEST40
pCMV SPORT6.1
pDONR Zeo
pEXP4-DEST
pcDNA-DEST47
pCOLA-2-DEST
pEF-DEST51
pHELLSGATE
pcDNA-DEST53
pCOLA-3-DEST
pEF5 FRT V5-DEST
pHELLSGATE 12
pcDNA3.1 nV5-DEST
pDEST10
pENTR11
pHELLSGATE 4
pcDNA3.2 V5-DEST
pDEST14
pENTR1A
pHELLSGATE 8
pcDNA3.2 capTEV-CT V5-DEST
pDEST15
pENTR2B
pIB V5-His-DEST
pcDNA3.2 capTEV-NT V5-DEST
pDEST17
pENTR3C
pLenti4 BLOCK-iT-DEST
pcDNA6.2 C-EmGFP-DEST
pDEST20
pENTR4
pLenti4 V5-DEST
pcDNA6.2 C-YFP-DEST
pDEST24
pENTR GeneBLAzer
pLenti6.2 C-Lumio V5-DEST
pcDNA6.2 EmGFP-Bsd V5-DEST
pDEST26
pET-53-DEST
pLenti6.2 N-Lumio V5-DEST
pcDNA6.2 N-EmGFP-DEST
pDEST27
pET-54-DEST
pLenti6.2 V5-DEST
pcDNA6.2 N-YFP-DEST
pDEST8
pET-55-DEST
pLenti6.3 TO V5-DEST
pcDNA6.2 V5-DEST
pDEST R4-R3
pET-56-DEST
pLenti6.3 V5-DEST
pcDNA6.2 V5-pL-DEST
pDEST R4-R3 Vector II
pET-57-DEST
pLenti6.4 R4R2 V5-DEST
pcDNA6.2 cGeneBLAzer-DEST
pDONR201
pET-58-DEST
pLenti6 BLOCK-iT-DEST
pcDNA6.2 cLumio-DEST
pDONR207
pET-59-DEST
pLenti6 UbC V5-DEST
pcDNA6.2 nGeneBLAzer-DEST
pDONR221
pET-60-DEST
pLenti6 V5-DEST
pcDNA6.2 nLumio-DEST
pDONR221 P1-P4
pET-61-DEST
pLenti6 capTEV-CT-DEST
pDONR221 P1-P5r
pET-62-DEST
pLenti6 capTEV-NT-DEST1

Plasmid Sets

Basic Cloning Vectors
Gateway® Cloning Vectors
pGEX Vectors (GE Healthcare)
TA and GC Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
TOPO Cloning Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Viral Expression & Packaging Vectors
I.M.A.G.E. Consortium Plasmids
Mammalian Expression Vectors
Structural Genomics Vectors
Yeast Plasmids
Insect Cell Vectors
pET & Duet Vectors (Novagen)

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ‘‘www.snapgene.com/resources‘‘. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience

Try for Free