pEU-GST-FV

Flexi® Vector with the barnase gene, for in vitro transcription and translation of a protein with an N-terminal GST-HRV 3C cassette.

Sequence Author: Center for Eukaryotic Structural Genomics

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Structural Genomics Vectors | More Plasmid Sets
No matches
BsrGI (5549) NaeI (5356) NgoMIV (5354) SgrAI (5345) AfeI (5263) PfoI (4964) FspI (4153) BglI (4051) BsaI (3992) AhdI (3931) AlwNI (3454) BsgI (438) SwaI (579) BclI * (586) AsiSI - SgfI (840) Bpu10I (1288) BspEI (1511) PasI - PflMI * (1747) PmeI (1966) Eco53kI (1979) SacI (1981) Acc65I (1983) KpnI (1987) XbaI (1998) SalI (2004) AccI (2005) HincII (2006) PstI - SbfI (2014) BlpI (2079) SpeI (2097) PshAI (2127) XcmI (2387) NsiI (2435) PmlI (2474) BsiWI - SnaBI (2481) BssHII (2538) HindIII (2679) pEU-GST-FV 5612 bp
BsrGI  (5549)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
NaeI  (5356)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (5354)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SgrAI  (5345)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AfeI  (5263)
1 site
A G C G C T T C G C G A
PfoI  (4964)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
FspI  (4153)
1 site
T G C G C A A C G C G T
BglI  (4051)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsaI  (3992)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3931)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (3454)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BsgI  (438)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SwaI  (579)
1 site
A T T T A A A T T A A A T T T A

SwaI is typically used at 25°C, but is 50% active at 37°C.
BclI  (586)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
AsiSI  (840)
1 site
G C G A T C G C C G C T A G C G
SgfI  (840)
1 site
G C G A T C G C C G C T A G C G
Bpu10I  (1288)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BspEI  (1511)
1 site
T C C G G A A G G C C T
PasI  (1747)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PflMI  (1747)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
PmeI  (1966)
1 site
G T T T A A A C C A A A T T T G
Eco53kI  (1979)
1 site
G A G C T C C T C G A G
SacI  (1981)
1 site
G A G C T C C T C G A G
Acc65I  (1983)
1 site
G G T A C C C C A T G G
KpnI  (1987)
1 site
G G T A C C C C A T G G
XbaI  (1998)
1 site
T C T A G A A G A T C T
SalI  (2004)
1 site
G T C G A C C A G C T G
AccI  (2005)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (2006)
1 site
G T Y R A C C A R Y T G
PstI  (2014)
1 site
C T G C A G G A C G T C
SbfI  (2014)
1 site
C C T G C A G G G G A C G T C C
BlpI  (2079)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
SpeI  (2097)
1 site
A C T A G T T G A T C A
PshAI  (2127)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
XcmI  (2387)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
NsiI  (2435)
1 site
A T G C A T T A C G T A
PmlI  (2474)
1 site
C A C G T G G T G C A C
BsiWI  (2481)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
SnaBI  (2481)
1 site
T A C G T A A T G C A T
BssHII  (2538)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
HindIII  (2679)
1 site
A A G C T T T T C G A A
AmpR
3858 .. 4718  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3858 .. 4649  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3858 .. 4718  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4650 .. 4718  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3858 .. 4718  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
GST
149 .. 805  =  657 bp
219 amino acids  =  25.6 kDa
Product: glutathione S-transferase from Schistosoma japonicum
GST
149 .. 805  =  657 bp
219 amino acids  =  25.6 kDa
Product: glutathione S-transferase from Schistosoma japonicum
HRV 3C site
812 .. 835  =  24 bp
8 amino acids  =  902.1 Da
Product: recognition and cleavage site for human rhinovirus 3C and PreScission proteases
HRV 3C site
812 .. 835  =  24 bp
8 amino acids  =  902.1 Da
Product: recognition and cleavage site for human rhinovirus 3C and PreScission proteases
CmR
1302 .. 1961  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
1302 .. 1961  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
3099 .. 3687  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3099 .. 3687  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
barnase
867 .. 1202  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
barnase
867 .. 1202  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
AmpR promoter
4719 .. 4823  =  105 bp
AmpR promoter
4719 .. 4823  =  105 bp
lac UV5 promoter
1218 .. 1248  =  31 bp
3 segments
   Segment 1:  -35  
   1218 .. 1223  =  6 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
1218 .. 1248  =  31 bp
3 segments
   Segment 2:  
   1224 .. 1241  =  18 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
1218 .. 1248  =  31 bp
3 segments
   Segment 3:  -10  
   1242 .. 1248  =  7 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
1218 .. 1248  =  31 bp
3 segments
E. coli lac promoter with an "up" mutation
SP6 promoter
1 .. 19  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
1 .. 19  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
ORF:  751 .. 1155  =  405 bp
ORF:  134 amino acids  =  14.7 kDa
ORF:  3988 .. 4254  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  149 .. 853  =  705 bp
ORF:  234 amino acids  =  27.2 kDa
ORF:  5195 .. 5485  =  291 bp
ORF:  96 amino acids  =  10.2 kDa
ORF:  867 .. 1202  =  336 bp
ORF:  111 amino acids  =  12.5 kDa
ORF:  1302 .. 1961  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  3858 .. 4718  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  5060 .. 5422  =  363 bp
ORF:  120 amino acids  =  12.2 kDa
ORF:  589 .. 846  =  258 bp
ORF:  85 amino acids  =  9.4 kDa
Click here to try SnapGene

Download pEU-GST-FV.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.