pBINPLUS

Improved binary Agrobacterium vector for plant transformation.
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EcoNI (10,768) PacI (9595) EcoRI (9585) SacI (9583) Eco53kI (9581) KpnI (9577) Acc65I (9573) SmaI (9571) TspMI - XmaI (9569) BamHI (9564) XbaI (9558) SalI (9552) SbfI (9550) HindIII (9534) AscI (9527) lac operator XmnI (9230) BspDI - ClaI (9108) MauBI (8817) AflII (8622) ApaI (8474) PspOMI (8470) BstBI (8219) BmtI (7290) NheI (7286) Bsu36I (7271) AhdI (6401) BbvCI (6343) NotI (92) PasI (645) PshAI (1412) MluI (1649) BstEII (1752) ApaLI (1852) BstXI (2686) SfiI (4318) PflMI * (4647) NdeI (4876) AleI (5002) MfeI (5272) FspAI (5658) NruI (6155) KflI (6253) pBINPLUS 12,396 bp
EcoNI  (10,768)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PacI  (9595)
1 site
T T A A T T A A A A T T A A T T
EcoRI  (9585)
1 site
G A A T T C C T T A A G
SacI  (9583)
1 site
G A G C T C C T C G A G
Eco53kI  (9581)
1 site
G A G C T C C T C G A G
KpnI  (9577)
1 site
G G T A C C C C A T G G
Acc65I  (9573)
1 site
G G T A C C C C A T G G
SmaI  (9571)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (9569)
1 site
C C C G G G G G G C C C
XmaI  (9569)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
BamHI  (9564)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (9558)
1 site
T C T A G A A G A T C T
SalI  (9552)
1 site
G T C G A C C A G C T G
SbfI  (9550)
1 site
C C T G C A G G G G A C G T C C
HindIII  (9534)
1 site
A A G C T T T T C G A A
AscI  (9527)
1 site
G G C G C G C C C C G C G C G G
XmnI  (9230)
1 site
G A A N N N N T T C C T T N N N N A A G
BspDI  (9108)
1 site
A T C G A T T A G C T A
ClaI  (9108)
1 site
A T C G A T T A G C T A
MauBI  (8817)
1 site
C G C G C G C G G C G C G C G C
AflII  (8622)
1 site
C T T A A G G A A T T C
ApaI  (8474)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (8470)
1 site
G G G C C C C C C G G G
BstBI  (8219)
1 site
T T C G A A A A G C T T
BmtI  (7290)
1 site
G C T A G C C G A T C G
NheI  (7286)
1 site
G C T A G C C G A T C G
Bsu36I  (7271)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
AhdI  (6401)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BbvCI  (6343)
1 site
C C T C A G C G G A G T C G
NotI  (92)
1 site
G C G G C C G C C G C C G G C G
PasI  (645)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PshAI  (1412)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
MluI  (1649)
1 site
A C G C G T T G C G C A
BstEII  (1752)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
ApaLI  (1852)
1 site
G T G C A C C A C G T G
BstXI  (2686)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.