pCAMBIA1303

Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance and GUS-GFP genes.

Sequence Author: Cambia

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Plant Vectors | More Plasmid Sets
No matches
NcoI (0) HindIII (11,600) PstI - SbfI (11,592) SalI (11,582) XbaI (11,576) BamHI (11,570) KpnI (11,565) Acc65I (11,561) BanII - SacI (11,559) Eco53kI (11,557) EcoRI (11,549) lac operator lac promoter BstXI (11,306) AatII (10,465) ZraI (10,463) AsiSI (10,126) RsrII (10,077) PspXI (9425) SacII (8907) PsiI (8779) Bpu10I (8634) BlpI (8303) PluTI (6771) SfoI (6769) NarI (6768) KasI (6767) MreI - SgrAI (6764) EcoNI (6295) BglII (7) SpeI (14) SnaBI (401) Bsu36I (2091) 6xHis BstEII (2573) MauBI (2819) RB T-DNA repeat PasI (4312) AclI (4906) BsiWI (5866) pCAMBIA1303 12,362 bp
NcoI  (0)
1 site
C C A T G G G G T A C C
HindIII  (11,600)
1 site
A A G C T T T T C G A A
PstI  (11,592)
1 site
C T G C A G G A C G T C
SbfI  (11,592)
1 site
C C T G C A G G G G A C G T C C
SalI  (11,582)
1 site
G T C G A C C A G C T G
XbaI  (11,576)
1 site
T C T A G A A G A T C T
BamHI  (11,570)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
KpnI  (11,565)
1 site
G G T A C C C C A T G G
Acc65I  (11,561)
1 site
G G T A C C C C A T G G
BanII  (11,559)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (11,559)
1 site
G A G C T C C T C G A G
Eco53kI  (11,557)
1 site
G A G C T C C T C G A G
EcoRI  (11,549)
1 site
G A A T T C C T T A A G
BstXI  (11,306)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
AatII  (10,465)
1 site
G A C G T C C T G C A G
ZraI  (10,463)
1 site
G A C G T C C T G C A G
AsiSI  (10,126)
1 site
G C G A T C G C C G C T A G C G
RsrII  (10,077)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PspXI  (9425)
1 site
V C T C G A G B B G A G C T C V
SacII  (8907)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PsiI  (8779)
1 site
T T A T A A A A T A T T
Bpu10I  (8634)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BlpI  (8303)
1 site
G C T N A G