pCAMBIA1303

Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance and GUS-GFP genes.

Sequence Author: Cambia

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NcoI (0) HindIII (11,600) PstI - SbfI (11,592) SalI (11,582) XbaI (11,576) BamHI (11,570) KpnI (11,565) Acc65I (11,561) BanII - SacI (11,559) Eco53kI (11,557) EcoRI (11,549) lac operator lac promoter BstXI (11,306) AatII (10,465) ZraI (10,463) AsiSI (10,126) RsrII (10,077) PspXI (9425) SacII (8907) PsiI (8779) Bpu10I (8634) BlpI (8303) PluTI (6771) SfoI (6769) NarI (6768) KasI (6767) MreI - SgrAI (6764) EcoNI (6295) BglII (7) SpeI (14) SnaBI (401) Bsu36I (2091) 6xHis BstEII (2573) MauBI (2819) RB T-DNA repeat PasI (4312) AclI (4906) BsiWI (5866) pCAMBIA1303 12,362 bp
NcoI  (0)
1 site
C C A T G G G G T A C C
HindIII  (11,600)
1 site
A A G C T T T T C G A A
PstI  (11,592)
1 site
C T G C A G G A C G T C
SbfI  (11,592)
1 site
C C T G C A G G G G A C G T C C
SalI  (11,582)
1 site
G T C G A C C A G C T G
XbaI  (11,576)
1 site
T C T A G A A G A T C T
BamHI  (11,570)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
KpnI  (11,565)
1 site
G G T A C C C C A T G G
Acc65I  (11,561)
1 site
G G T A C C C C A T G G
BanII  (11,559)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SacI  (11,559)
1 site
G A G C T C C T C G A G
Eco53kI  (11,557)
1 site
G A G C T C C T C G A G
EcoRI  (11,549)
1 site
G A A T T C C T T A A G
BstXI  (11,306)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
AatII  (10,465)
1 site
G A C G T C C T G C A G
ZraI  (10,463)
1 site