pCAMBIA2300

Agrobacterium binary vector for plant transformation, with kanamycin-resistance genes.

Sequence Author: Cambia

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PmeI (8623) HindIII (8410) PstI - SbfI (8402) SalI (8392) XbaI (8386) BamHI (8380) SmaI (8377) KpnI - TspMI - XmaI (8375) Acc65I (8371) SacI (8369) Eco53kI (8367) EcoRI (8359) lac operator BstXI (8116) BglII (7316) NcoI (7301) BssHII (6771) RsrII (6653) PspXI (6451) BclI * (5936) SacII (5933) PsiI (5805) Bpu10I (5660) EcoO109I - PpuMI (5521) BspHI (5479) BlpI (5329) SspI (4983) NsiI (4945) BsaBI * (1331) PasI (1338) AclI (1932) BsiWI (2892) NheI (3008) BmtI (3012) EcoNI (3321) BsaI (3411) AgeI (3502) MreI - SgrAI (3790) BstZ17I (3992) pCAMBIA2300 8743 bp
PmeI  (8623)
1 site
G T T T A A A C C A A A T T T G
HindIII  (8410)
1 site
A A G C T T T T C G A A
PstI  (8402)
1 site
C T G C A G G A C G T C
SbfI  (8402)
1 site
C C T G C A G G G G A C G T C C
SalI  (8392)
1 site
G T C G A C C A G C T G
XbaI  (8386)
1 site
T C T A G A A G A T C T
BamHI  (8380)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (8377)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
KpnI  (8375)
1 site
G G T A C C C C A T G G
TspMI  (8375)
1 site
C C C G G G G G G C C C
XmaI  (8375)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
Acc65I  (8371)
1 site
G G T A C C C C A T G G
SacI  (8369)
1 site
G A G C T C C T C G A G
Eco53kI  (8367)
1 site
G A G C T C C T C G A G
EcoRI  (8359)
1 site
G A A T T C C T T A A G
BstXI  (8116)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BglII  (7316)
1 site
A G A T C T T C T A G A
NcoI  (7301)
1 site
C C A T G G G G T A C C
BssHII  (6771)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
RsrII  (6653)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PspXI  (6451)
1 site
V C T C G A G B B G A G C T C V
BclI  (5936)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
SacII  (5933)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PsiI  (5805)
1 site
T T A T A A A A T A T T
Bpu10I  (5660)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
EcoO109I  (5521)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (5521)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
BspHI  (5479)
1 site
T C A T G A A G T A C T
BlpI  (5329)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
SspI  (4983)
1 site
A A T A T T T T A T A A
NsiI  (4945)
1 site
A T G C A T T A C