pSB1

Superbinary acceptor vector for plant cell transformation, with a tetracycline-resistance gene.
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KpnI (45) AvrII (20,349) MauBI (19,473) virD1 SnaBI (5139) FseI (8462) BsiWI (11,188) KpnI (14,854) BstZ17I (15,048) PciI (15,275) SbfI (16,851) pSB1 36,909 bp
KpnI  (45)
2 sites
G G T A C C C C A T G G
AvrII  (20,349)
1 site
C C T A G G G G A T C C
MauBI  (19,473)
1 site
C G C G C G C G G C G C G C G C
SnaBI  (5139)
1 site
T A C G T A A T G C A T
FseI  (8462)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
BsiWI  (11,188)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
KpnI  (14,854)
2 sites
G G T A C C C C A T G G
BstZ17I  (15,048)
1 site
G T A T A C C A T A T G
PciI  (15,275)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
SbfI  (16,851)
1 site
C C T G C A G G G G A C G T C C
incC,korABFG operon
31,395 .. 34,828  =  3434 bp
incC,korABFG operon
31,395 .. 34,828  =  3434 bp
virB4
9057 .. 11,426  =  2370 bp
789 amino acids  =  87.4 kDa
Product: VirB4
integral inner membrane ATPase
virB4
9057 .. 11,426  =  2370 bp
789 amino acids  =  87.4 kDa
Product: VirB4
integral inner membrane ATPase
kfrBC operon
28,439 .. 30,352  =  1914 bp
kfrBC operon
28,439 .. 30,352  =  1914 bp
TcR
19,088 .. 20,287  =  1200 bp
399 amino acids  =  42.2 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
TcR
19,088 .. 20,287  =  1200 bp
399 amino acids  =  42.2 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
trfA
21,560 .. 22,708  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds to and activates oriV
trfA
21,560 .. 22,708  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds to and activates oriV
virB10
4487 .. 5620  =  1134 bp
377 amino acids  =  40.5 kDa
Product: VirB10
T-DNA transfer
virB10
4487 .. 5620  =  1134 bp
377 amino acids  =  40.5 kDa
Product: VirB10
T-DNA transfer
virB11
3416 .. 4447  =  1032 bp
343 amino acids  =  38.0 kDa
Product: VirB11
T-DNA transfer
virB11
3416 .. 4447  =  1032 bp
343 amino acids  =  38.0 kDa
Product: VirB11
T-DNA transfer
virB6
7396 .. 8283  =  888 bp
295 amino acids  =  31.8 kDa
Product: VirB6
T-DNA transfer
virB6
7396 .. 8283  =  888 bp
295 amino acids  =  31.8 kDa
Product: VirB6
T-DNA transfer
virG
2481 .. 3284  =  804 bp
267 amino acids  =  30.0 kDa
Product: VirG
response regulator of virA/virG two component transduction system
virG
2481 .. 3284  =  804 bp
267 amino acids  =  30.0 kDa
Product: VirG
response regulator of virA/virG two component transduction system
virB1
12,132 .. 12,851  =  720 bp
239 amino acids  =  25.9 kDa
Product: VirB1
T-DNA transfer
virB1
12,132 .. 12,851  =  720 bp
239 amino acids  =  25.9 kDa
Product: VirB1
T-DNA transfer
virB8
6495 .. 7208  =  714 bp
237 amino acids  =  26.2 kDa
Product: VirB8
T-DNA transfer
virB8
6495 .. 7208  =  714 bp
237 amino acids  =  26.2 kDa
Product: VirB8
T-DNA transfer
oriV
35,754 .. 36,465  =  712 bp
incP origin of replication
oriV
35,754 .. 36,465  =  712 bp
incP origin of replication
virC1 (inactivated)
1065 .. 1759  =  695 bp
Product: VirC1, inactivated by a frameshift mutation that eliminated the final C in codon Thr-56
virC1 (inactivated)
1065 .. 1759  =  695 bp
Product: VirC1, inactivated by a frameshift mutation that eliminated the final C in codon Thr-56
virB5
8380 .. 9042  =  663 bp
220 amino acids  =  23.5 kDa
Product: VirB5
T-DNA transfer
virB5
8380 .. 9042  =  663 bp
220 amino acids  =  23.5 kDa
Product: VirB5
T-DNA transfer
TetR
18,332 .. 18,982  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
TetR
18,332 .. 18,982  =  651 bp
216 amino acids  =  23.3 kDa
Product: tetracycline resistance regulatory protein
virC2
1762 .. 2370  =  609 bp
202 amino acids  =  22.4 kDa
Product: VirC2
virC2
1762 .. 2370  =  609 bp
202 amino acids  =  22.4 kDa
Product: VirC2
ori
15,336 .. 15,924  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
15,336 .. 15,924  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
virD1
353 .. 796  =  444 bp
147 amino acids  =  16.3 kDa
Product: VirD1
cleavage accessory protein
virD1
353 .. 796  =  444 bp
147 amino acids  =  16.3 kDa
Product: VirD1
cleavage accessory protein
cos
16,432 .. 16,830  =  399 bp
lambda cos site; allows packaging into phage lambda particles
cos
16,432 .. 16,830  =  399 bp
lambda cos site; allows packaging into phage lambda particles
traJ
26,118 .. 26,489  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
traJ
26,118 .. 26,489  =  372 bp
123 amino acids  =  13.5 kDa
Product: oriT-recognizing protein
virB2
11,752 .. 12,117  =  366 bp
121 amino acids  =  12.3 kDa
Product: VirB2
pilin protein precursor
virB2
11,752 .. 12,117  =  366 bp
121 amino acids  =  12.3 kDa
Product: VirB2
pilin protein precursor
bom
15,010 .. 15,150  =  141 bp
basis of mobility region from pBR322
bom
15,010 .. 15,150  =  141 bp
basis of mobility region from pBR322
oriT
26,522 .. 26,631  =  110 bp
incP origin of transfer
oriT
26,522 .. 26,631  =  110 bp
incP origin of transfer
kfrA operon
30,253 .. 31,335  =  1083 bp
kfrA operon
30,253 .. 31,335  =  1083 bp
virB9
5617 .. 6498  =  882 bp
293 amino acids  =  32.1 kDa
Product: VirB9
T-DNA transfer
virB9
5617 .. 6498  =  882 bp
293 amino acids  =  32.1 kDa
Product: VirB9
T-DNA transfer
virB3
11,426 .. 11,752  =  327 bp
108 amino acids  =  11.8 kDa
Product: VirB3
T-DNA transfer
virB3
11,426 .. 11,752  =  327 bp
108 amino acids  =  11.8 kDa
Product: VirB3
T-DNA transfer
virB7
7195 .. 7362  =  168 bp
55 amino acids  =  5.8 kDa
Product: VirB7
T-DNA transfer
virB7
7195 .. 7362  =  168 bp
55 amino acids  =  5.8 kDa
Product: VirB7
T-DNA transfer
ORF:  1762 .. 2370  =  609 bp
ORF:  202 amino acids  =  22.4 kDa
ORF:  2662 .. 3294  =  633 bp
ORF:  210 amino acids  =  23.3 kDa
ORF:  4837 .. 5082  =  246 bp
ORF:  81 amino acids  =  9.5 kDa
ORF:  5491 .. 5718  =  228 bp
ORF:  75 amino acids  =  8.5 kDa
ORF:  8521 .. 8799  =  279 bp
ORF:  92 amino acids  =  10.6 kDa
ORF:  9559 .. 9813  =  255 bp
ORF:  84 amino acids  =  9.1 kDa
ORF:  11,674 .. 11,898  =  225 bp
ORF:  74 amino acids  =  8.0 kDa
ORF:  13,138 .. 13,518  =  381 bp
ORF:  126 amino acids  =  14.6 kDa
ORF:  16,225 .. 16,536  =  312 bp
ORF:  103 amino acids  =  11.0 kDa
ORF:  25,276 .. 25,587  =  312 bp
ORF:  103 amino acids  =  12.1 kDa
ORF:  26,857 .. 27,261  =  405 bp
ORF:  134 amino acids  =  14.7 kDa
ORF:  28,936 .. 29,199  =  264 bp
ORF:  87 amino acids  =  9.0 kDa
ORF:  29,506 .. 29,811  =  306 bp
ORF:  101 amino acids  =  10.6 kDa
ORF:  34,261 .. 34,488  =  228 bp
ORF:  75 amino acids  =  8.2 kDa
ORF:  1202 .. 1759  =  558 bp
ORF:  185 amino acids  =  20.9 kDa
ORF:  2147 .. 2449  =  303 bp
ORF:  100 amino acids  =  11.5 kDa
ORF:  4856 .. 5332  =  477 bp
ORF:  158 amino acids  =  17.5 kDa
ORF:  11,798 .. 12,382  =  585 bp
ORF:  194 amino acids  =  21.7 kDa
ORF:  13,301 .. 13,633  =  333 bp
ORF:  110 amino acids  =  12.4 kDa
ORF:  16,826 .. 17,062  =  237 bp
ORF:  78 amino acids  =  8.9 kDa
ORF:  19,013 .. 20,287  =  1275 bp
ORF:  424 amino acids  =  45.1 kDa
ORF:  21,572 .. 21,967  =  396 bp
ORF:  131 amino acids  =  14.7 kDa
ORF:  23,282 .. 23,593  =  312 bp
ORF:  103 amino acids  =  11.3 kDa
ORF:  23,927 .. 24,823  =  897 bp
ORF:  298 amino acids  =  32.5 kDa
ORF:  24,836 .. 25,273  =  438 bp
ORF:  145 amino acids  =  15.0 kDa
ORF:  25,448 .. 25,747  =  300 bp
ORF:  99 amino acids  =  11.2 kDa
ORF:  26,990 .. 27,457  =  468 bp
ORF:  155 amino acids  =  17.4 kDa
ORF:  27,983 .. 28,420  =  438 bp
ORF:  145 amino acids  =  15.6 kDa
ORF:&#