pGreenII 0579

Agrobacterium binary vector with kanamycin- resistance and luciferase genes.

Sequence Author: pGreen website

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DraIII (5538) NruI (5347) PasI (5129) SspI (5079) AsiSI (5006) Bpu10I - BsmBI (4984) PflMI (4744) AlwNI (4217) ApaLI (4115) DrdI (3909) BglII (3796) StuI (3743) lac operator SacI (3340) Eco53kI (3338) SacII (3331) AleI - MslI (3330) NotI (3319) XbaI (3312) SpeI (3306) BamHI (3300) SmaI (3296) TspMI - XmaI (3294) PstI (3292) EcoRV (3279) HindIII (3271) AccI (3257) SalI (3256) AbsI - PaeR7I - PspXI - XhoI (3250) ApaI (3245) PspOMI (3241) KpnI (3239) Acc65I (3235) FspI (3064) BsaBI * (62) StyI (421) NgoMIV (524) NaeI (526) FseI (528) AanI (546) BglII (627) LB T-DNA repeat ScaI (677) BtgZI (918) SgrAI (1174) BfuAI - BspMI (1204) SexAI * (1262) PacI (1284) PpuMI (1424) XcmI (1872) NspI - SphI (1947) Bsu36I (1990) BstEII (1994) AflIII (2109) BsrGI (2112) AanI (2281) BsiWI (2448) PfoI * (2494) KasI (2570) NarI (2571) SfoI (2572) BbeI (2574) BmgBI (2738) PshAI (2801) pGreenII 0579 5664 bp
DraIII  (5538)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
NruI  (5347)
1 site
T C G C G A A G C G C T
PasI  (5129)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
SspI  (5079)
1 site
A A T A T T T T A T A A
AsiSI  (5006)
1 site
G C G A T C G C C G C T A G C G
Bpu10I  (4984)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsmBI  (4984)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
PflMI  (4744)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AlwNI  (4217)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
ApaLI  (4115)
1 site
G T G C A C C A C G T G
DrdI  (3909)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BglII  (3796)
2 sites
A G A T C T T C T A G A
StuI  (3743)
1 site
A G G C C T T C C G G A
SacI  (3340)
1 site
G A G C T C C T C G A G
Eco53kI  (3338)
1 site
G A G C T C C T C G A G
SacII  (3331)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
AleI  (3330)
1 site
C A C N N N N G T G G T G N N N N C A C
MslI  (3330)
1 site
C A Y N N N N R T G G T R N N N N Y A C
NotI  (3319)
1 site
G C G G C C G C C G C C G G C G
XbaI  (3312)
1 site
T C T A G A A G A T C T
SpeI  (3306)
1 site
A C T A G T T G A T C A
BamHI  (3300)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (3296)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (3294)
1 site
C C C G G G G G G C C C
XmaI  (3294)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
PstI  (3292)
1 site
C T G C A G G A C G T C
EcoRV  (3279)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
HindIII  (3271)
1 site
A A G C T T T T C G A A
AccI  (3257)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (3256)
1 site
G T C G A C C A G C T G
AbsI  (3250)
1 site
C C T C G A G G G G A G C T C C
PaeR7I  (3250)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (3250)
1 site
V C T C G A G B